Analysis of T cell antigen receptor (TCR) expression by human peripheral blood CD4-8- alpha/beta T cells demonstrates preferential use of several V beta genes and an invariant TCR alpha chain.
Analysis of T cell antigen receptor (TCR) expression by human peripheral blood CD4-8- alpha/beta T cells demonstrates preferential use of several V beta genes and an invariant TCR alpha chain.
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DOI：
10.1084/jem.178.1.1
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发表时间：
1993-07-01
期刊：
影响因子：
--
通讯作者：
Balk SP
中科院分区：
文献类型：
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作者：
Porcelli S;Yockey CE;Brenner MB;Balk SP
CD4-CD8-（双阴性[DN]） α / β T细胞是一个功能未知的亚群。为了研究这些细胞是否被选择识别特定抗原或抗原呈递分子，从5个正常供者的外周血中纯化DN α / β T细胞，并检测其T细胞受体(TCR) α和β链。通过单侧聚合酶链反应（PCR）随机克隆TCR α链，发现V α 24和J α Q之间存在不变的重排，没有N区多样性，在所有供体的DN α / β T细胞中优先表达。随机克隆还发现了一个精确的V α 7.2-J α (IGRJa14)重排，在V- j连接编码了两个可变氨基酸，在一些（但不是全部）供体的DN α / β T细胞制备中富集。通过定量PCR扩增对TCR β链的分析表明，在这些DN α / β T细胞制备中，V β 2、8、11和13四个V β基因家族的表达显著增加。来自多个供体的DN α / β T细胞对特定tcr的表达表明，这些细胞，或至少具有这种表型的细胞亚群，识别有限的抗原谱，并表明它们可能使用非多态性抗原提呈分子。
CD4-CD8- (double negative [DN]) alpha/beta T cells are a largely uncharacterized subpopulation of unknown function. To investigate whether these cells are selected to recognize particular antigens or antigen-presenting molecules, DN alpha/beta T cells were purified from the peripheral blood of five normal donors and their T cell receptor (TCR) alpha and beta chains were examined. Random cloning of TCR alpha chains by single-sided polymerase chain reaction (PCR) amplification identified an invariant rearrangement between V alpha 24 and J alpha Q, with no N region diversity, which was expressed preferentially by DN alpha/beta T cells from all donors. Random cloning also identified a precise V alpha 7.2-J alpha (IGRJa14) rearrangement, with two variable amino acids encoded in the V-J junction, which was enriched in the DN alpha/beta T cell preparations from some, but not all, donors. Analysis of TCR beta chains by quantitative PCR amplification demonstrated that the expression of four V beta gene families, V beta 2, 8, 11, and 13, was markedly increased in these DN alpha/beta T cell preparations. The expression of particular TCRs by DN alpha/beta T cells from multiple donors indicates that these cells, or at least a subpopulation of cells with this phenotype, recognize a limited spectrum of antigens and suggests that they may use nonpolymorphic antigen-presenting molecules.