Soft Palate Modification Using a Collagen Crosslinking Reagent for Equine Dorsal Displacement of the Soft Palate and Other Upper Airway Breathing Disorders.
Soft Palate Modification Using a Collagen Crosslinking Reagent for Equine Dorsal Displacement of the Soft Palate and Other Upper Airway Breathing Disorders.
复制标题
使用胶原交联试剂对马软腭背侧移位和其他上呼吸道呼吸疾病进行软腭修饰。
DOI：
10.1155/2019/9310890
复制
发表时间：
2019
影响因子：
3.1
通讯作者：
Hedman,Thomas
中科院分区：
文献类型：
--
作者：
Hunt,Stephanie;Kuo,Jonathan;Aristizabal,FabioA;Brown,Matt;Patwardhan,Abhijit;Hedman,Thomas
软腭的机械特性可能与呼吸异常有关。软腭背侧移位（DDSP）是一种自然发生的马软腭疾病，由软腭尾侧缘移位引起。打鼾和更严重的，有时危及生命的，称为阻塞性睡眠呼吸暂停（OSA）的疾病是人类睡眠相关呼吸障碍的形式，可能涉及软腭。本研究的目的是调查的效果注入蛋白质交联剂京尼平到软腭，以修改其机械性能的治疗马DDSP与潜在的影响，打鼾和OSA的治疗在human.Ex vivoexperiments包括机械测试和风洞研究，以检查京尼平对马软腭的机械性能，位移和振动的影响。使用DDSP和对照马完成了一项pilotin体内研究，以测试将京尼平试剂注射到软腭中的安全性和有效性。风洞试验表明，对于所有试验剂量的京尼平，瞬态变形降低大于50%，稳态振动降低大于33%。京尼平处理的远端软腭样本的极限拉伸应力、屈服应力和杨氏模量分别高出52%、53%和63%。pilotin体内研究显示，所有DDSP马的打鼾响度降低，三匹马中至少有一匹马的DDSP消除。难以使用1米长的内窥镜注射针导致马软腭持续过度注射，导致过度拉伸（枕状）和相关组织降解。这些体内和体内结果表明，京尼平处理的软腭的振动幅度和松弛度降低，强度增加，表明京尼平交联可能成为软腭相关呼吸异常的有效和安全的治疗方法。
The mechanical properties of the soft palate can be associated with breathing abnormalities. Dorsal displacement of the soft palate (DDSP) is a naturally occurring equine soft palate disorder caused by displacement of the caudal edge of the soft palate. Snoring and a more serious, sometimes life‐threatening, condition called obstructive sleep apnea (OSA) are forms of sleep‐related breathing disorders in humans which may involve the soft palate. The goal of this study was to investigate the effect of injecting the protein crosslinker genipin into the soft palate to modify its mechanical properties for the treatment of equine DDSP with potential implications for the treatment of snoring and OSA in humans.Ex vivoexperiments consisted of mechanical testing and a wind tunnel study to examine the effect of genipin on the mechanical properties, displacement, and vibration of equine soft palates. A pilotin vivostudy was completed using DDSP and control horses to test the safety and effectiveness of injecting a genipin reagent into the soft palate. The wind tunnel testing demonstrated a greater than 50% decrease in transient deformation and a greater than 33% decrease in steady‐state vibrations for all doses of genipin tested. Ultimate tensile stress, yield stress, and Young’s modulus were higher in the genipin‐treated distal soft palate specimens by 52%, 53%, and 63%, respectively. The pilotin vivostudy showed a reduction of snoring loudness in all DDSP horses and elimination of DDSP in at least one of three horses. The difficulty of using a 1‐meter‐long endoscopic injection needle contributed to a consistent overinjection of the equine soft palates, causing excessive stretching (pillowing) and related degradation of the tissue. Theseex vivoandin vivoresults demonstrated reduced vibration amplitude and flaccidity and increased strength of genipin‐treated soft palates, suggesting that genipin crosslinking could become an effective and safe treatment for soft palate related breathing abnormalities.