The O139 serogroup of Vibrio cholerae comprises diverse clones of epidemic and nonepidemic strains derived from multiple V. cholerae O1 or non-O1 progenitors
The O139 serogroup of Vibrio cholerae comprises diverse clones of epidemic and nonepidemic strains derived from multiple V. cholerae O1 or non-O1 progenitors
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DOI：
10.1086/315807
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发表时间：
2000-10-01
影响因子：
6.4
通讯作者：
Nair, GB
中科院分区：
文献类型：
--
作者：
Faruque, SM;Saha, MN;Nair, GB
对64株具有代表性的霍乱弧菌O139进行了分析，对该血清群的起源进行了重新研究，通过核型分型将菌株区分为3种HindIII和7种BglI核型，所有产毒株O139共有1种HindIII和5种BglI核型。在6株非产毒株中，3株与产毒株具有相同的核型，携带毒素协同调节菌毛编码基因，对霍乱毒素转化噬菌体CTX Phi敏感。其余3株菌株分别属于2种不同于产毒株的核型，均对CTX Phi和O139特异性裂解噬菌体JA-1具有抗性，其携带的gmhD基因聚合酶链反应扩增子也与产毒株不同，但与15株环境非o1 -非O139菌株相同。因此，O139抗原存在于不同的谱系中，该血清群似乎包括分别来自不同祖细胞的流行株和非流行株。
Sixty-four representative strains of Vibrio cholerae O139 were analyzed, to re-examine the origin of this serogroup, Ribotyping differentiated the strains into 3 HindIII and 7 BglI ribotypes, One HindIII and 5 BglI ribotypes were shared by all toxigenic O139 strains. Of 6 nontoxigenic O139 strains, 3 shared ribotypes with the toxigenic strains, carried genes encoding toxin coregulated pilus, and were susceptible to the cholera toxin-converting bacteriophage CTX Phi. The remaining 3 strains belonged to 2 different ribotypes distinct from toxigenic O139 strains and were resistant to CTX Phi, and JA-1, an O139-specific lytic bacteriophage, Polymerase chain reaction amplicons corresponding to the gmhD gene carried by these 3 strains also differed from those of the toxigenic O139 strains but were identical to those of 15 environmental non-O1-non-O139 strains. Thus, the O139 antigen is present in different lineages, and this serogroup appears to comprise epidemic and nonepidemic strains derived separately from different progenitors.