T cell mediation of abnormally low production of ovalbumin-specific immunoglobulin A in patients allergic to eggs
T cell mediation of abnormally low production of ovalbumin-specific immunoglobulin A in patients allergic to eggs
复制标题
DOI：
10.1203/00006450-199602000-00023
复制
发表时间：
1996-02-01
影响因子：
3.6
通讯作者：
Baba, M
中科院分区：
文献类型：
--
作者：
Noma, T;Yamaguchi, K;Baba, M
用斑块形成细胞（PFC）检测产生卵清蛋白（OVA）特异性IgA的细胞。在聚苯乙烯树脂柱上分离非t细胞，并通过SRBC-玫瑰花沉降进一步去除与羊红细胞结合的B细胞。将这些细胞与聚苯乙烯树脂柱分离的T细胞重组，用OVA抗原刺激，培养5 d。鸡蛋过敏婴儿淋巴细胞中OVA特异性IgA-PFC的数量（每7 × 10(4)个非T细胞7 +/- 5个，n = 9）显著少于年龄匹配对照组淋巴细胞的PFC数量（每7 × 10(4)个非T细胞110 +/- 18个）。n = 7)和对鸡蛋不过敏的特应性皮炎儿童（90 +/- 30 / 7 × 10(4)个非t细胞，n = 4）。患者的B细胞添加到文化上层清液从OVA-stimulated正常T细胞(每7 X 10 82 + / - 18 (4) non-T细胞,n = 4)能够产生特定的IgA同等的水平正常B细胞(92 + / - 9 / 7 X 10 (4) non-T细胞,n = 6),但病人的T细胞并没有导致正常B细胞产生抗体(8 + / - 2 / 7 X 10 (4) non-T细胞,n = 4),这表明患者的T细胞比正常T细胞更不能促进ova特异性IgA-PFC的产生。直到6岁，患者淋巴细胞产生特异性IgA的能力异常低；从那个年龄开始，这就很正常了。在过敏原进入的阶段，这种短暂的低ova特异性IgA的产生可能有助于对鸡蛋过敏的发生。
Cells producing IgA specific to ovalbumin (OVA) were detected with an assay of plaque-forming cells (PFC). Non-T cells were separated on a polystyrene resin column and were further depleted of B cells that bound sheep erythrocytes (SRBC) by SRBC-rosette sedimentation. The cells were recombined with T cells separated on a polystyrene resin column, stimulated with OVA antigen, and then cultured for 5 d. The number of OVA-specific IgA-PFC from the lymphocytes of infants allergic to hen's eggs (7 +/- 5 per 7 X 10(4) non-T cells, n = 9) was significantly less than that of PFC from lymphocytes of age-matched controls (110 +/- 18 per 7 X 10(4) non-T cells, n = 7) and from those of children with atopic dermatitis who were not allergic to hen's eggs (90 +/- 30 per 7 x 10(4) non-T cells, n = 4). Patients' B cells added to the culture supernatant from OVA-stimulated normal T cells (82 +/- 18 per 7 X 10(4) non-T cells, n = 4) were able to produce the specific IgA to levels comparable to those of normal B cells (92 +/- 9 per 7 X 10(4) non-T cells, n = 6), but the patients' T cells did not cause normal B cells to produce the antibody (8 +/- 2 per 7 X 10(4) non-T cells, n = 4), This indicates that the patients' T cells were less able than were normal T cells to promote the production of OVA-specific IgA-PFC. Until the age of 6 y, the ability of the patients' lymphocytes to produce specific IgA was abnormally low; from that age on, it was normal. At the stage of allergen entry, this transiently low production of OVA-specific IgA may contribute to the onset of allergy to hen's eggs.