Ionic liquid‐assisted SDS‐PAGE to improve human serum protein separation
Ionic liquid‐assisted SDS‐PAGE to improve human serum protein separation
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DOI：
10.1002/elps.201100184
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发表时间：
2011-10
期刊：
影响因子：
2.9
通讯作者：
Tao Zhang;Qingqing Gai;Feng Qu;Yukui Zhang
中科院分区：
文献类型：
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作者：
Tao Zhang;Qingqing Gai;Feng Qu;Yukui Zhang
采用离子液体(IL)辅助十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(ILS-SDS-PAGE)进行蛋白质分离。在聚丙烯酰胺凝胶的制备过程中加入离子液体，将其用于商业蛋白标记物、牛血清白蛋白/溶菌酶(BSA/LYZ)和人血清的分离。考察了离子液体浓度、阳离子烷基链长、阳离子类型和阴离子类型对蛋白质分离的影响。结果表明，ILS对人血清中低相对分子质量蛋白质的分离起到了一定的促进作用，ILS-SDS-PAGE对人血清中低相对分子质量蛋白质的分离度和分离效率高于普通的SDS-PAGE。此外，还对ILS-SDS-PAGE的原理进行了探讨，并与普通的SDS-PAGE和Native-PAGE进行了比较。
Ionic liquid (IL)‐assisted sodium dodecyl sulfate polyacrylamide gel electrophoresis (ILs‐SDS‐PAGE) was presented to improve protein separation. ILs were employed during the preparation process of polyacrylamide gel, then the modified gel was used for commercial protein marker, binary bovine serum albumin/lysozyme (BSA/Lyz) and human serum separation. The influence of ionic liquid concentration, cation alkyl chain length, cation and anion types on proteins separation were investigated. The results showed that ILs played a role in improving some protein separation, and ILs‐SDS‐PAGE provided higher resolution and separation efficiency than ordinary SDS‐PAGE for low and middle relative molecular mass proteins in human serum. In addition, the principle of ILs‐SDS‐PAGE was discussed and the comparison of ILs‐SDS‐PAGE with ordinary SDS‐PAGE and Native PAGE was made.