Effect of lead exposure on the immune response of some occupationally exposed individuals
Effect of lead exposure on the immune response of some occupationally exposed individuals
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DOI：
10.1016/s0300-483x(03)00091-x
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发表时间：
2003-06-30
期刊：
影响因子：
4.5
通讯作者：
Seth, PK
中科院分区：
文献类型：
--
作者：
Mishra, KP;Singh, VK;Seth, PK
铅是工业环境中普遍存在的污染物，对人类健康构成严重威胁。在过去的20年里，人们越来越关注铅暴露对健康的影响。这种有毒金属会改变动物和人类的免疫反应。为了研究职业性铅暴露的免疫学效应，我们检测了职业性铅接触者外周血单个核细胞(PBMC)的淋巴细胞增殖、自然杀伤细胞(NK)细胞毒性和干扰素-γ的产生。我们选择了三组不同的铅接触者：三轮车司机(30人)、电池工人(34人)和银饰制造商(20人)；以及未接触铅的健康志愿者(30人)作为对照。我们的结果表明，尽管与非铅接触者相比，铅接触者淋巴细胞对植物血凝素(PHA)的增殖受到抑制，但淋巴细胞增殖抑制与血铅水平没有相关性。与对照组相比，铅暴露组的NK细胞杀伤活性没有受到影响。另一方面，我们观察到铅接触者T细胞有丝分裂原、PHA刺激的PBMC培养上清液中的干扰素-γ水平显著升高。血铅水平与PHA刺激培养上清液中产生的干扰素-γ呈显著正相关。简而言之，这项研究表明，铅可以影响职业接触者的免疫反应，如三轮车司机、电池修理工和银器首饰制造商。(C)2003爱思唯尔爱尔兰科学有限公司。保留所有权利。
Lead is a ubiquitous pollutant in the industrial environment, which poses serious threats to human health. In the past 20 years increasing attention has been paid to the effects of lead exposure on health. This toxic metal alters the immune response of animals as well as humans. To study the immunological effects of occupational exposure to lead, we examined lymphocyte proliferation, natural killer (NK) cell cytotoxicity and interferon-gamma production with peripheral blood mononuclear cells (PBMCs) of individuals occupationally exposed to lead. We selected three different groups of individuals exposed to lead: three-wheeler drivers (30), battery workers (34) and silver jewelery makers (20); and unexposed healthy volunteers (30) as control for comparison. Our results indicate that though lymphocyte proliferation to phytohaemagglutinin (PHA) is inhibited in lead exposed individuals as compared with unexposed volunteers, there is no correlation between inhibition of lymphocyte proliferation and blood lead level. NK cell cytotoxicity remains unaffected in individuals exposed to lead as compared with controls. On the other hand, we observed that interferon-gamma (IFN-gamma) was significantly elevated in T cell mitogen, PHA, stimulated PBMCs culture supernatant of lead exposed individuals. We found significant positive correlation between blood lead levels and IFN-gamma produced in culture supernatant on stimulation with PHA. In brief, this study demonstrates that lead can affect the immune response of the occupationally exposed individuals such as three-wheeler drivers, battery reconditioning workers and silver jewelery makers. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.