Dorsoventral patterning in xenopus: Inhibition of ventral signals by direct binding of Chordin to BMP-4
Dorsoventral patterning in xenopus: Inhibition of ventral signals by direct binding of Chordin to BMP-4
复制标题
DOI：
10.1016/s0092-8674(00)80132-4
复制
发表时间：
1996-08-23
期刊：
影响因子：
64.5
通讯作者：
DeRobertis, EM
中科院分区：
文献类型：
--
作者：
Piccolo, S;Sasai, Y;DeRobertis, EM
Chordin（Chd）是原肠胚形成过程中Spemann组织细胞分泌的一种丰富的蛋白质。Chd通过阻断与其受体的结合来拮抗成熟骨形态发生蛋白（BMP）的信号传导。重组非洲爪蟾Chd以高亲和力（K-D 3 × 10（-10）M）结合BMP-4，特异性结合BMP，但不结合激活素或TGF-β 1。Chd蛋白在1 nM时能够使非洲爪蟾原肠胚外植体中的中胚层背化和外胚层神经化。我们建议，Spemann的组织者背腹图案的非细胞自主的影响执行部分由可扩散的信号，直接绑定到和中和腹侧骨形成蛋白在原肠胚形成。
Chordin (Chd) is an abundant protein secreted by Spemann organizer tissue during gastrulation. Chd antagonizes signaling by mature bone morphogenetic proteins (BMPs) by blocking binding to their receptors. Recombinant Xenopus Chd binds to BMP-4 with high affinity (K-D 3 x 10(-10) M), binding specifically to BMPs but not to activin or TGF-beta 1. Chd protein is able to dorsalize mesoderm and to neuralize ectoderm in Xenopus gastrula explants at 1 nM. We propose that the noncell-autonomous effects of Spemann's organizer on dorsoventral patterning are executed in part by diffusible signals that directly bind to and neutralize ventral BMPs during gastrulation.