The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS
The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS
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DOI：
10.1038/s41467-020-16082-2
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发表时间：
2020-05-01
影响因子：
16.6
通讯作者：
Buratowski, Stephen
中科院分区：
文献类型：
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作者：
Bae, Hyun Jin;Dubarry, Marion;Buratowski, Stephen
Set1/COMPASS对组蛋白H3赖氨酸4 （H3K4）的甲基化发生共转录，对基因调控很重要。Set1/COMPASS与RNA聚合酶II c端结构域（CTD）相关，以建立适当的H3K4甲基化水平和分布。然而，CTD关联的细节仍不清楚。本文报道了Set1 n端区域和COMPASS亚基Swd2相互作用，它们都是酿酒酵母有效结合CTD所必需的。此外，Swd2中影响其与Set1相互作用的单点突变也会损害COMPASS向染色质和H3K4甲基化的募集。来自Nrd1蛋白的CTD相互作用域（CID）可以部分替代Set1 n端区域，以恢复CTD相互作用和组蛋白甲基化。然而，即使Set1/COMPASS通过Nrd1 CID募集，有效的H3K4甲基化仍然需要组蛋白H2B泛素化，这表明H2Bub在COMPASS最初募集到染色质后起作用。Set1/COMPASS对组蛋白H3赖氨酸4 （H3K4）的甲基化发生共转录，对基因调控很重要。在这里，作者提供了证据，证明Set1 n端区域和COMPASS亚基Swd2与RNA聚合酶II CTD相互作用，在出芽酵母中招募COMPASS。
Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally, and is important for gene regulation. Set1/COMPASS associates with the RNA polymerase II C-terminal domain (CTD) to establish proper levels and distribution of H3K4 methylations. However, details of CTD association remain unclear. Here we report that the Set1 N-terminal region and the COMPASS subunit Swd2, which interact with each other, are both needed for efficient CTD binding in Saccharomyces cerevisiae. Moreover, a single point mutation in Swd2 that affects its interaction with Set1 also impairs COMPASS recruitment to chromatin and H3K4 methylation. A CTD interaction domain (CID) from the protein Nrd1 can partially substitute for the Set1 N-terminal region to restore CTD interactions and histone methylation. However, even when Set1/COMPASS is recruited via the Nrd1 CID, histone H2B ubiquitylation is still required for efficient H3K4 methylation, indicating that H2Bub acts after the initial recruitment of COMPASS to chromatin. Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally, and is important for gene regulation. Here the authors provide evidence that the Set1 N-terminal region and the COMPASS subunit Swd2 interact with RNA polymerase II CTD to recruit COMPASS in budding yeast.