Insulin gene expression pattern in rat pancreas during the estrous cycle
Insulin gene expression pattern in rat pancreas during the estrous cycle
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DOI：
10.1016/s0024-3205(01)01100-6
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发表时间：
2001-05-18
期刊：
影响因子：
6.1
通讯作者：
Díaz-Sánchez, V
中科院分区：
文献类型：
--
作者：
Morimoto, S;Cerbón, MA;Díaz-Sánchez, V
性激素影响胰岛素稳态和糖代谢，雌二醇(E-2)和孕酮(P-4)可引起啮齿类动物空腹和餐后胰岛素血症的改变，但胰岛素基因在动情周期中的表达尚不清楚。本研究的目的是确定大鼠发情周期中胰岛素基因的表达模式。发情周期每组6只成年大鼠。用放射免疫法(RIA)测定血清P-4、E-2、睾酮(T)和胰岛素浓度。Northern杂交分析胰岛素基因在胰腺组织中的表达。我们发现，在发情周期中，胰岛素基因的表达有明显的变化。在发情前期，胰岛素的表达最高。有趣的是，E-2和P-4水平与胰岛素mRNA含量的变化相关，而与T水平无关。血清胰岛素在周期中的变化与其在胰腺中的mRNA含量相关。总体结果显示，大鼠发情周期中血清胰岛素和胰岛素基因表达的变化与循环中的E-2和P-4水平相关。(C)2001 Elsevier Science Inc.保留所有权利。
Sex steroid hormones influence insulin homeostasis and glucose metabolism, estradiol (E-2) and progesterone (P-4) induce changes in both fasting and postprandial insulinemia in rodents, however, insulin gene expression during estrous cycle is unknown. The aim of the present study was to determine an insulin gene expression pattern during the estrous cycle in the rat. Groups of 6 adult rats in each day of the estrous cycle were used. Serum P-4, E-2, testosterone (T) and insulin concentrations were determined by radioimmunoassay (RIA). A Northern blot analysis was performed to assess insulin gene expression in pancreatic tissue. We found a marked variation in insulin gene expression during the estrous cycle. The highest insulin expression was observed during the proestrus day. Interestingly, E-2 and P-4 but not T levels were correlated with changes in insulin mRNA content. The variations in serum insulin during the cycle were correlated with its mRNA content in pancreas. The overall results showed variations in serum insulin and insulin gene expression during estrous cycle of the rat that correlated with circulating E-2 and P-4 levels. (C) 2001 Elsevier Science Inc. All rights reserved.