The human homologue of the Drosophila tailless gene (TLX):: Characterization and mapping to a region of common deletion in human lymphoid leukemia on chromosome 6q21
The human homologue of the Drosophila tailless gene (TLX):: Characterization and mapping to a region of common deletion in human lymphoid leukemia on chromosome 6q21
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DOI：
10.1006/geno.1998.5270
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发表时间：
1998-05-15
期刊：
影响因子：
4.4
通讯作者：
Foroni, L
中科院分区：
文献类型：
--
作者：
Jackson, A;Panayiotidis, P;Foroni, L
6号染色体长臂（6 q）缺失是人类恶性血液病中最常见的染色体异常之一。通过杂合性缺失研究，在6 q25至6 q27（RMD-1）和6 q21至6 q23（RMD-2）发现了两个不同的最小缺失区域，表明存在一个或多个肿瘤抑制基因。我们克隆了RMD-2内的序列，并使用直接测序、cDNA文库筛选和外显子捕获的组合筛选新基因。从粘粒片段产生的序列，映射RMD-2内，显示同源性的果蝇无尾基因（tll）。果蝇无尾基因的人类同源物（人类tlx; MGMW批准的符号，TLX）随后从胎儿脑cDNA文库中克隆。该基因是类固醇核受体超家族的成员，并且与来自其他参与大脑发育的物种的tll基因同源。TLX主要在脑中表达，并且在也含有标记D 6S 246的YAC克隆中，在DNA标记FYN和D 6S 447之间的6 q21处映射到RMD-2。该基因对人类B细胞白血病和大脑发育的贡献目前尚不清楚。(C)北京：科学出版社.
Deletion of the long arm of chromosome 6 (6q) is one of the most common chromosomal abnormalities in human hematological malignancies. Two distinct regions of minimal deletion have been identified by loss of heterozygosity studies at 6q25 to 6q27 (RMD-1) and at 6q21 to 6q23 (RMD-2), suggesting the presence of one or more tumor suppressor genes. We have cloned sequences within RMD-2 and screened for novel genes using a combination of direct sequencing, cDNA library screening, and exon trapping. Sequences generated from a cosmid fragment, mapping within RMD-2, showed homology to the Drosophila tailless gene (tll). The human homologue of the Drosophila tailless gene (human tlx; MGMW-approved symbol, TLX) was subsequently cloned from a fetal brain cDNA library. The gene is a member of the steroid nuclear receptor superfamily and is homologous to tll genes fi om other species that are involved in brain development. TLX is predominately expressed in the brain and maps to RMD-2 at 6q21 between DNA markers FYN and D6S447, in a YAC clone that also contains marker D6S246. The contributions of this gene to human B-cell leukemia and to brain development are unknown at present. (C) 1998 Academic Press.