Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.
Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes.
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荧光团封装的固体支撑双层囊泡：一种研究膜渗透过程的方法。
DOI：
10.1021/la060853q
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
A. Jenkins
中科院分区：
文献类型：
--
作者：
Thomas L. Williams;Margarida M L M Vareiro;A. Jenkins
本文介绍了一种新的研究膜裂解酶磷脂酶A（2）（PLA（2））与磷脂双分子层相互作用的方法，即用表面等离子体共振（SPR）同时测量酶结合和囊泡裂解，用表面等离子体场增强荧光光谱（SPFS）同时测量透化作用。将聚乳酸（2）抑制剂二甲基二十碳二烯酸掺入囊泡和支持双层的表面结合，以研究其在防止聚乳酸（2）介导的囊泡溶解中的作用。这种方法具有一系列的膜破坏剂的研究的一般适用性。
This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.