The neural cell adhesion molecule binds to fibroblast growth factor receptor 2
The neural cell adhesion molecule binds to fibroblast growth factor receptor 2
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DOI：
10.1016/j.febslet.2006.05.008
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发表时间：
2006-06-12
期刊：
影响因子：
3.5
通讯作者：
Kiselyov, Vladislav V.
中科院分区：
文献类型：
--
作者：
Christensen, Claus;Lauridsen, Jes B.;Kiselyov, Vladislav V.
神经细胞粘附分子（NCAM）可以结合并激活成纤维细胞生长因子受体1（FGFR 1）。然而，有四种主要的FGFR亚型（FGFR 1-FGFR 4），目前尚不清楚NCAM是否也与其他三种FGFR亚型直接相互作用。在这项研究中，我们通过表面等离子体共振分析表明，NCAM可以结合FGFR 2的亲和力类似于NCAM-FGFR 1的相互作用。然而，NCAM-FGFR 2结合的动力学参数与NCAM-FGFR 1结合的动力学参数不同。两种受体在NCAM结合和未结合状态之间的循环相对较快，尽管FGFR 2循环明显快于FGFR 1循环（13倍）。此外，ATP在抑制NCAM与FGFR 1的结合方面比与FGFR 2的结合更有效，表明NCAM中两种受体的结合位点相似，但不相同。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The neural cell adhesion molecule (NCAM) can bind to and activate fibroblast growth factor receptor 1 (FGFR1). However, there are four major FGFR isoforms (FGFR1-FGFR4), and it is not known whether NCAM also interacts directly with the other three FGFR isoforms. In this study, we show by surface plasmon resonance analysis that NCAM can bind to FGFR2 with an affinity similar to that for the NCAM-FGFR1 interaction. However, the kinetic parameters for the NCAM-FGFR2 binding are different from those of the NCAM-FGFR1 binding. Both receptors were shown to cycle relatively fast between the NCAM bound and unbound states, although FGFR2 cycling was clearly faster (13 times) than the FGFR1 cycling. Moreover, ATP was more effective in inhibiting the binding of NCAM to FGFR1 than to FGFR2, indicating that the binding sites in NCAM for the two receptors are similar, but not identical. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.