Analysis of Nitrification in Agricultural Soil and Improvement of Nitrogen Circulation with Autotrophic Ammonia-Oxidizing Bacteria
Analysis of Nitrification in Agricultural Soil and Improvement of Nitrogen Circulation with Autotrophic Ammonia-Oxidizing Bacteria
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DOI：
10.1007/s12010-012-0029-6
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发表时间：
2013-02-01
影响因子：
3
通讯作者：
Kubo, Motoki
中科院分区：
文献类型：
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作者：
Matsuno, Toshihide;Horii, Sachie;Kubo, Motoki
通过分析无机氮（NH4（+）、NO2（-）和NO3（-））的累积量来评估76个农业土壤的氮循环活性。观察到 NH4 (+) 的积累，农田土壤中 NH4 (+) → aEuro 千分之一 NO2 (-) 的反应似乎比 NO2 (-) → aEuro 千分之一 NO3 (-) 的反应慢。从土壤中分离鉴定出2种自养型和5种异养型氨氧化细菌(AOB)，自养型AOB的氨氧化活性比异养型AOB高1.0×10(3)-1.0×10(6)倍。对 30 个农业土壤的 AOB 数、土壤细菌数和氨氧化活性之间的关系进行了研究。自养AOB数占土壤细菌总数的比例为0.00032-0.26%。富含自养AOB（>1.0 x 10(4) cells/g土壤）的土壤样品具有较高的氮循环活性，并且通过控制自养AOB改善了农业土壤的氮循环。
Accumulations of inorganic nitrogen (NH4 (+), NO2 (-), and NO3 (-)) were analyzed to evaluate the nitrogen circulation activity in 76 agricultural soils. Accumulation of NH4 (+) was observed, and the reaction of NH4 (+) -> aEuro parts per thousand NO2 (-) appeared to be slower than that of NO2 (-) -> aEuro parts per thousand NO3 (-) in agricultural soil. Two autotrophic and five heterotrophic ammonia-oxidizing bacteria (AOB) were isolated and identified from the soils, and the ammonia-oxidizing activities of the autotrophic AOB were 1.0 x 10(3)-1.0 x 10(6) times higher than those of heterotrophic AOB. The relationship between AOB number, soil bacterial number, and ammonia-oxidizing activity was investigated with 30 agricultural soils. The ratio of autotrophic AOB number was 0.00032-0.26 % of the total soil bacterial number. The soil samples rich in autotrophic AOB (> 1.0 x 10(4) cells/g soil) had a high nitrogen circulation activity, and additionally, the nitrogen circulation in the agricultural soil was improved by controlling the autotrophic AOBs.