Effects of Oscillatory Flow on the Nucleation and Crystallization of Insulin
Effects of Oscillatory Flow on the Nucleation and Crystallization of Insulin
复制标题
振荡流对胰岛素成核和结晶的影响
DOI：
10.1021/cg200158z
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发表时间：
2011
影响因子：
3.8
通讯作者：
Parambil J
中科院分区：
文献类型：
--
作者：
Parambil J
蛋白质结晶具有巨大的潜力，可用于工业规模的分离和配方应用。静态条件下的结晶常常受到分子从体溶液向生长的晶体表面扩散的限制。这导致产品的总收率较低，结晶周期较长。因此，流动条件下的蛋白质结晶由于能够改善蛋白质的对流运输，缩短结晶时间，提高产量而受到人们的关注。在这项研究中，我们研究了流动，特别是间歇振荡流动对蛋白质（胰岛素）结晶的影响。我们观察到，流动条件对胰岛素结晶的成核和产率有很大的影响，本文提出了在间歇振荡流动下进行胰岛素结晶的一些关键观察结果。在振荡流速范围为6 ~ 16mm /min，频率为1循环/min的情况下，与固定管相比，随着流速的增加，成核率显著增加，导致大量晶体在流动管中形成。在48小时内，流动结晶的产率达到50%，而固定生长的产率只有24%。因此，流动条件会影响晶体的数量和大小。此外，本研究中采用的间歇流动模式有助于分别了解流动对成核和晶体生长的影响。本文提出的流动策略有可能用于优化蛋白质的结晶过程，从而用于下游产品的分离和配方。
Protein crystallization has immense potential to be used for separation and formulation applications on an industrial scale. Crystallization under static conditions is often limited by the diffusion of molecules from the bulk solution to the growing crystal surface. This results in a lower overall yield of the product and longer crystallization periods. Hence, protein crystallization under flow conditions has attracted attention with the capability to improve the convective protein transport, reducing the crystallization time and improving yield. In this study, we investigate the effects of flow, specifically intermittent oscillatory flow on protein (insulin) crystallization. It was observed that the nucleation and crystal yield is very much influenced by the flow conditions and herein we present some key observations in insulin crystallization conducted under intermittent oscillatory flow. For oscillatory flow velocities ranging from 6 to 16 mm/min at a frequency of 1 cycle/min, a considerable increase in nucleation has been observed with an increase in flow rate resulting in the formation of large number of crystals in tubes with flow compared to the stationary tubes. Also 50% yield for flow crystallization was achieved in contrast to only 24% yield for the stationary growth over the 48 h period. The flow condition was thus found to affect the number and size of crystals. In addition, the intermittent flow pattern utilized in this study helps to separately understand the influence of flow on nucleation and crystal growth. The flow strategy proposed herein could potentially be utilized to optimize crystallization processes for proteins so as to be used in downstream separation and formulation of products.
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DOI：
--
发表时间：
2007
期刊：
影响因子：
--
作者：
R. Ristic
通讯作者：
R. Ristic
微重力下生物大分子的结晶：一种获得更精确三维结构的方法？
DOI：
--
发表时间：
2002
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
B. Lorber
通讯作者：
B. Lorber
通过玻璃毛细管强制流动进行蛋白质结晶：增强溶菌酶晶体生长
影响因子：
3.8
作者：
Roberts M
通讯作者：
Roberts M
蛋白质结晶筛选中采样方案的效率分析
影响因子：
1.8
作者：
B. Segelke
通讯作者：
B. Segelke
DOI：
--
发表时间：
2009
期刊：
影响因子：
--
作者：
D. Schruben;Julien A. Stephanus;María Elena González
通讯作者：
María Elena González