Nuclear matrix contains novel WD-repeat and disordered-region-rich proteins
Nuclear matrix contains novel WD-repeat and disordered-region-rich proteins
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DOI：
10.1016/j.febslet.2008.09.019
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发表时间：
2008-10-15
期刊：
影响因子：
3.5
通讯作者：
Horigome, Tsuneyoshi
中科院分区：
文献类型：
--
作者：
Ishii, Kohei;Hirano, Yasuhiro;Horigome, Tsuneyoshi
要找到新的蛋白质预测参与形成的核小体，核斑点，和核宏蛋白复合物，我们应用蛋白质组分析的HeLa细胞核基质馏分。通过SDS-PAGE分离组分中的蛋白质，用胰蛋白酶消化，并通过纳流液相色谱-离子阱-串联质谱分析。共鉴定出333个蛋白质，其中39个为新蛋白质。在新的蛋白质中发现了7个WD重复蛋白和16个富含无序区域的蛋白质，它们经常作为大蛋白复合物的支架蛋白。
To find novel proteins predicted to participate in the formation of nuclear bodies, nuclear speckles, and nuclear macro-protein complexes, we applied proteome analysis to a HeLa cell nuclear matrix fraction. Proteins in the fraction were separated by SDS-PAGE, digested with trypsin, and analyzed by nanoflow liquid chromatography-iontrap-tandem mass spectrometry. Three hundred and thirty three proteins including 39 novel ones were identified. Seven WD-repeat proteins and 16 disordered region-rich proteins, which act frequently as scaffholding proteins for macro-protein complexes, were found amongst the novel proteins.