Particle size determines the accumulation of platinum nanoparticles in the estuarine amphipod, Leptocheirus plumulosus
Particle size determines the accumulation of platinum nanoparticles in the estuarine amphipod, Leptocheirus plumulosus
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粒径决定铂纳米颗粒在河口端足类动物 Leptocheirus penulosus 中的积累
DOI：
10.1039/d1en00713k
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Baalousha, Mohammed
中科院分区：
文献类型：
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作者：
Sikder, Mithun;Eudy, Emily;Cai, Bo;Chandler, G. Thomas;Baalousha, Mohammed
纳米颗粒（NPs）通常在水生环境中显示出不同大小的广泛分布，但关于粒径分散对生物体吸收和消除的影响的信息很少。本研究研究了不同尺寸（20.0±4.8 nm, 40.5±4.1 nm和70.8±4.2 nm）的聚乙烯吡咯烷酮包覆铂纳米颗粒（PVP-PtNPs）在口端脚类毛羽细尾绦虫（Leptocheirus plumlosus）体内的吸收和消除。利用电感耦合等离子体质谱法测定暴露于PtNPs的片脚类动物体内Pt的总负荷，并利用单粒子icp - ms （sp-ICP-MS）测定PtNPs体内Pt的质量和数量，从而确定Pt的积累和消除。L. plumlosus从不同大小的PtNP悬浮液中积累了Pt，大多数（例如bbb90 %）以PtNP的形式而不是溶解的Pt。随着PtNP大小的减少，基于质量和数量的吸收增加，而随着PtNP大小的增加，基于质量和数量的消除增加。随着PtNPs大小的减小，PtNPs消除48 h后的残余全身负荷增加，暴露于20 nm PtNPs的片脚类动物的残余体负荷大约是暴露于70 nm PtNPs的片脚类动物的两倍。PtNP内流率（kuw）随NP尺寸的减小而增加，20 nm、40 nm和70 nm PtNP内流率分别为1.07±0.31、0.82±0.22和0.67±0.10 μg−1 d−1。PtNP流出率（ke）随PtNP尺寸的增大而增大，20 nm、40 nm和70 nm PtNP流出率分别为0.31±0.08、0.66±0.04和0.83±0.07 d−1。当暴露于40 nm和70 nm PtNPs的质量、表面积或40 nm和70 nm PtNPs数量浓度相等的混合物时，L. plumlosus积累的40 nm PtNPs数量高于所有混合物中70 nm PtNPs的数量。混合物中70 nm PtNPs暴露浓度的增加不影响40 nm PtNPs的摄取，这表明在多分散NP悬浮液中，给定尺寸部分的摄取与混合物中其他尺寸部分无关。
Nanoparticles (NPs) typically display a wide distribution of different sizes in aquatic environments, yet little information is available on the impact of particle size dispersity on organismal uptake and elimination. This study investigated uptake and elimination of polyvinylpyrrolidone-coated platinum nanoparticles (PVP-PtNPs) of different sizes (e.g., 20.0 ± 4.8 nm, 40.5 ± 4.1 nm, and 70.8 ± 4.2 nm) by the estuarine amphipod Leptocheirus plumulosus. Accumulation and elimination were determined by measuring total Pt body burden in amphipods exposed to PtNPs using inductively coupled plasma-mass spectroscopy, as well as the mass and number PtNP body burden using single particle-ICP-MS (sp-ICP-MS). L. plumulosus accumulated Pt from PtNP suspensions of different sizes from water exposure, mostly (e.g., >90%) as PtNPs rather than as dissolved Pt. Mass- and number-based uptake increased with decreases in PtNP size whereas mass- and number-based elimination increased with increasing PtNP size. The residual whole-animal body burden of PtNPs after 48 h elimination increased with decreases in PtNP size, with residual body burdens approximately two-fold higher for amphipods exposed to 20 nm PtNPs than amphipods exposed to 70 nm PtNPs. PtNP influx rate (kuw) increased with decreasing NP size, with kuws of 1.07 ± 0.31, 0.82 ± 0.22, and 0.67 ± 0.10 μg g−1 d−1 for 20 nm, 40 nm, and 70 nm PtNPs, respectively. PtNP efflux rate (ke) increased with increasing PtNP size, with kes of 0.31 ± 0.08, 0.66 ± 0.04, and 0.83 ± 0.07 d−1 for 20 nm, 40 nm, and 70 nm PtNP, respectively. When exposed to mixtures of 40 and 70 nm PtNPs with equal masses, surface areas, or number concentrations of 40 nm and 70 nm PtNPs, L. plumulosus accumulated higher numbers of the 40 nm PtNPs than 70 nm PtNPs from all mixtures. The increased exposure concentration of 70 nm PtNPs in the mixture did not affect the uptake of 40 nm PtNPs, suggesting that in a polydispersed NP suspension the uptake of a given size fraction is independent of other size fractions in the mixture.