A novel biomarker for marine environmental pollution of HSP90 from Mytilus coruscus.
A novel biomarker for marine environmental pollution of HSP90 from Mytilus coruscus.
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DOI：
10.1016/j.marpolbul.2016.07.031
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发表时间：
2016-10
影响因子：
5.8
通讯作者：
Hui-hui Liu;Jiong Wu;Mengsha Xu;Jian-yu He
中科院分区：
文献类型：
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作者：
Hui-hui Liu;Jiong Wu;Mengsha Xu;Jian-yu He
热休克蛋白90（HSP 90）是一种保守的分子伴侣，参与细胞周期调控、生物体发育和胞浆蛋白的正常调节。贻贝HSP 90（McHSP 90，KT 946644）cDNA全长2420 bp，开放阅读框2169 bp，编码722个氨基酸，等电点/分子量为4.89/83.22 kDa。BLASTp分析和系统进化关系表明McHSP 90是HSP 90家族的一员，与其他已知的HSP 90高度保守，尤其是在HSP 90家族特征、ATP/GTP结合位点和EEVD基序上。McHSP 90 mRNA在溶藻弧菌和哈氏弧菌攻击、金属胁迫（铜和镉）和180 CST燃料暴露中均上调。上述结果提示，环境胁迫和弧菌攻击均能影响McHSP 90的表达，这为进一步研究HSP在贝类抗逆境胁迫中的分子机制提供了理论依据。
Heat shock protein 90 (HSP90) is a conserved molecular chaperone contributing to cell cycle control, organism development and the proper regulation of cytosolic proteins. The full-length HSP90 cDNA of Mytiluscoruscus(McHSP90, KT946644) was 2420 bp, including an ORF of 2169 bp encoding a polypeptide of 722 amino acids with predictedpI/MW 4.89/83.22 kDa. BLASTp analysis and phylogenetic relationship strongly suggested McHSP90 was a member of HSP90 family, and it was highly conserved with other known HSP90, especially in the HSP90 family signatures, ATP/GTP-Binding sites and ‘EEVD’ motif. The mRNA of McHSP90 in haemolymph was upregulated in all treatments including Vibrioalginolyticusand Vibrio harveyi challenge, metals stresses (copper and cadmium) and 180 CST fuel exposure. All the results implied the expression of McHSP90 could be affected byVibriochallenge and environmental stress, which might help us gain more insight into the molecular mechanism of HSP against adverse stresses in mollusca.