Natural Killer Cells for Immunotherapy - Advantages of the NK-92 Cell Line over Blood NK Cells.
Natural Killer Cells for Immunotherapy - Advantages of the NK-92 Cell Line over Blood NK Cells.
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DOI：
10.3389/fimmu.2016.00091
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发表时间：
2016
影响因子：
7.3
通讯作者：
Toneguzzo F
中科院分区：
文献类型：
--
作者：
Klingemann H;Boissel L;Toneguzzo F
自然杀伤（NK）细胞是用于癌症治疗和潜在地用于严重病毒感染的有效细胞毒性效应细胞。然而，从患者血液中获得足够数量的功能活性NK细胞存在技术挑战，因为它们仅占淋巴细胞的10%并且通常功能失调。另一种方法是从健康供体获得细胞，这需要耗尽同种异体T细胞以防止移植物抗宿主反应。细胞毒性细胞系已经从具有克隆性NK细胞淋巴瘤的患者中建立。这些细胞可以在IL-2存在下在培养物中扩增。然而，除了NK-92细胞系之外，其他六种已知的NK细胞系都没有一致地且可重复地显示出高的抗肿瘤细胞毒性。只有NK-92细胞可以很容易地进行遗传操作，以识别特定的肿瘤抗原或通过抗体依赖性细胞毒性增强单克隆抗体的活性。NK-92也是唯一一种已输注到晚期癌症患者体内的细胞系产品，具有临床益处和最小的副作用。
Natural killer (NK) cells are potent cytotoxic effector cells for cancer therapy and potentially for severe viral infections. However, there are technical challenges to obtain sufficient numbers of functionally active NK cells from a patient’s blood since they represent only 10% of the lymphocytes and are often dysfunctional. The alternative is to obtain cells from a healthy donor, which requires depletion of the allogeneic T cells to prevent graft-versus-host reactions. Cytotoxic cell lines have been established from patients with clonal NK-cell lymphoma. Those cells can be expanded in culture in the presence of IL-2. Except for the NK-92 cell line, though, none of the other six known NK cell lines has consistently and reproducibly shown high antitumor cytotoxicity. Only NK-92 cells can easily be genetically manipulated to recognize specific tumor antigens or to augment monoclonal antibody activity through antibody-dependent cellular cytotoxicity. NK-92 is also the only cell line product that has been infused into patients with advanced cancer with clinical benefit and minimal side effects.