Plasmalogens in the retina: In situ hybridization of dihydroxyacetone phosphate acyltransferase (DHAP-AT) - the first enzyme involved in their biosynthesis - and comparative study of retinal and retinal pigment epithelial lipid composition
Plasmalogens in the retina: In situ hybridization of dihydroxyacetone phosphate acyltransferase (DHAP-AT) - the first enzyme involved in their biosynthesis - and comparative study of retinal and retinal pigment epithelial lipid composition
复制标题
DOI：
10.1016/j.exer.2006.09.009
复制
发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Bretillon, Lionel
中科院分区：
文献类型：
--
作者：
Acar, Niyazi;Gregoire, Stephane;Bretillon, Lionel
血浆原(Pis)是一种在甘油主链的sn-1位含有乙烯基醚键的磷脂。PI的生理作用仍然是个谜，特别是在眼睛中，它们的缺乏会导致发育异常。为了更好地了解Pis在后眼中的功能，我们检测了视网膜PI含量以及参与Pis生物合成的第一个酶--二羟丙酮磷酸酰基转移酶(DHAP-AT)在视网膜中的表达。用大鼠眼部切片进行DHAP-AT基因的原位杂交。用高效液相色谱法、薄层层析法和气相色谱法测定了小牛视网膜和视网膜色素上皮(RPE)中PI的含量。DHAP-AT在光感受器内段和RPE中高表达，提示PI有两个不同的生物合成部位。质膜乙醇胺是神经视网膜和视网膜色素上皮中最重要的一类磷脂酰甘油酯，占总磷脂酰乙醇胺甘油三酯的28-29%。根据与Pls的sn-1位相连的烯基残基的性质，最令人震惊的发现是，与神经视网膜和RPE中所有其他类别的Pis相比，神经视网膜的质膜乙醇胺sn-1位的十八醛比例更大。这些发现可能与Pis抗氧化应激和形成脂筏的生物学功能有关。(C)2006爱思唯尔有限公司。保留所有权利。
Plasmalogens (Pis) are phospholipids containing a vinyl-ether bond in the sn-1 position of the glycerol backbone. The physiological role of PIs is still enigmatic, especially within the eye where their deficiency leads to developmental abnormalities. In order to learn more about the functions of Pis in the posterior eye, we evaluated retinal PI content as well as the expression of the first enzyme involved in Pis biosynthesis, dihydroxyacetone phosphate acyltransferase (DHAP-AT) in the retina. In situ hybridization of DHAP-AT mRNA was performed on rat eye sections. The PI contents of calf retina and retinal pigment epithelium (RPE) samples were determined by high-performance liquid chromatography, thin-layer chromatography, and gas chromatography. DHAP-AT was highly expressed in the inner segment of photoreceptors and in the RPE, suggesting two distinct sites for PI biosynthesis. Plasmenyl-ethanolamine was the prominent class of Pis in both neural retina and RPE (28-29% of the total phospho-ethanolamine-glycerides). According to the nature of the alkenyl residue linked to the sn-1 position of Pls, the most striking finding was the greater proportion of octadecanal-aidehyde in the sn-1 position of plasmenyl-ethanolamine of the neural retina compared to all the other classes of Pis in the neural retina and the RPE. These findings might be relevant to the biological functions of Pis against oxidative stress and in the formation of lipid rafts. (c) 2006 Elsevier Ltd. All rights reserved.