Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity
Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity
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DOI：
10.1016/s0092-8674(00)81615-3
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发表时间：
1998-11-13
期刊：
影响因子：
64.5
通讯作者：
Stein, D
中科院分区：
文献类型：
--
作者：
Sen, J;Goltz, JS;Stein, D
果蝇卵巢体细胞组织中pipe的表达是胚胎背腹极性形成所必需的。pipe编码一种类似于糖胺聚糖修饰酶硫酸乙酰肝素2-O-磺基转移酶的酶，在卵室的卵囊侧的卵泡细胞的空间限制域中表达。影响毛囊极性的突变相应地改变管道表达的空间模式。在其他管道突变的女性管道定向表达恢复胚胎的侧面和腹侧图案元素，并可以定向背腹轴的胚胎。因此，pipe的局部表达和糖链的空间限制性修饰在建立胚胎模式和整合卵泡和胚胎极性的机制中起着关键作用。
Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on the Ventral side of the egg chamber. Mutations that affect follicular polarity correspondingly alter the spatial pattern of pipe expression. Directed expression of pipe in otherwise pipe mutant females restores embryonic lateral and ventral pattern elements and can orient the dorsal-ventral axis of the embryo. Thus, the localized expression of pipe and the spatially restricted modification of carbohydrate chains play pivotal roles in the mechanisms that establish embryonic pattern and integrate follicular and embryonic polarity.