In vitro homogeneous and heterogeneous degradation of poly(ε-caprolactone/polyethylene glycol/L-lactide):: the absence of autocatalysis and the role of enzymes
In vitro homogeneous and heterogeneous degradation of poly(ε-caprolactone/polyethylene glycol/L-lactide):: the absence of autocatalysis and the role of enzymes
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DOI：
10.1002/jbm.a.30739
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发表时间：
2006-10-01
影响因子：
4.9
通讯作者：
Zhang, Ze
中科院分区：
文献类型：
--
作者：
Wang, Zhaoxu;Wang, Shenguo;Zhang, Ze
研究了聚（ε-己内酯/聚乙二醇/L-丙交酯）（PCEL）的体外降解行为，并与其他三种可生物降解聚合物进行了比较。将聚合物膜在胰酶溶液、林格氏溶液和蒸馏水中在37 ℃下孵育长达20周。表征包括测量重量损失，通过扫描电子显微镜（SEM）观察形态变化，使用尺寸排阻色谱法（SEC）分析分子量，以及使用差示扫描量热法（DSC）研究晶体结构。在简单水溶液中的水解没有经历自催化，这归因于PCEL对水溶性降解产物的高渗透性。PCEL和聚（L，L-丙交酯）（PLLA）试验膜记录了相似的降解速率。在胰酶的存在下，PCEL膜经历了快速的异质性表面侵蚀，可能是由酶促作用下其表面PEG组分的选择性损失引起的。胰酶还通过消除自催化作用而显著增加了其它测试聚合物的均匀物理再吸收。这项研究表明，聚（α-羟基酸）通常经历的自催化可以通过化学制剂或高酶活性来减少。(c)2006 Wiley Periodicals，Inc.
This study investigated the in vitro degradation behavior of poly(epsilon-caprolactone/polyethylene glycol/L-lactide) (PCEL) in comparison with that of three other biodegradable polymers. Polymer membranes were incubated in pancreatin solution, Ringer's solution, and distilled water at 37 degrees C for up to 20 weeks. Characterization involved measuring weight loss, observing the morphological changes by scanning electron microscopy (SEM), analyzing molecular weight using size exclusion chromatography (SEC), and studying the crystalline structure using differential scanning calorimetry (DSC). The hydrolysis in a simple aqueous solution experienced no autocatalysis, which was attributed to the high permeability of PCEL to water-soluble degradation products. Similar degradation rates were recorded for the PCEL and poly(L,L-lactide) (PLLA) test membranes. In the presence of pancreatin, the PCEL membrane experienced rapid heterogeneous surface erosion likely caused by the selective loss of its surface PEG components under enzymatic action. Pancreatin also substantially increased the even physical resorption of the other test polymers by eliminating autocatalysis. This study demonstrated that autocatalysis commonly experienced by poly(alpha-hydroxyl acid) can be reduced through chemical formulation or high enzyme activity. (c) 2006 Wiley Periodicals, Inc.