Molecular and biochemical detection of fengycin- and bacillomycin D-producing Bacillus spp., antagonistic to fungal pathogens of canola and wheat
Molecular and biochemical detection of fengycin- and bacillomycin D-producing Bacillus spp., antagonistic to fungal pathogens of canola and wheat
复制标题
对油菜和小麦真菌病原体产生拮抗作用的产风霉素和杆菌霉素 D 芽孢杆菌的分子和生化检测
DOI：
10.1139/w07-049
复制
发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
de Kievit, Teresa
中科院分区：
文献类型：
--
作者：
Ramarathnam, Rajesh;Bo, Shen;de Kievit, Teresa
芽孢杆菌属物种以其通过各种机制（包括产生次级代谢物）控制植物病害的能力而众所周知。枯草芽孢杆菌DFH 08，禾谷镰刀菌的拮抗剂，和其他芽孢杆菌。对油菜常见病原真菌的拮抗菌进行了丰霉素和芽孢杆菌霉素D肽合成酶基因的筛选。特异性聚合酶链反应（PCR）引物鉴定为B。枯草杆菌菌株DFH 08和49中fenD基因的存在。蜡样芽孢杆菌DFE 4、解淀粉芽孢杆菌菌株DFE 16和BS 6以及B。枯草芽孢杆菌49中鉴定出存在芽孢杆菌霉素D合成酶生物合成操纵子的bamC基因。通过基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption ionization - time of flight - mass spectrometry，MALDI-TOF-MS）对菌株Bs 49的发酵液进行了鉴定，并对菌株Bs 49的抑菌活性进行了研究。禾本科和核盘菌。本研究设计并使用了特异性PCR引物来检测潜在的产丰霉素和杆菌霉素D的细菌拮抗剂，并通过所产生的相应抗生素的生化检测来证实分子检测。这也是A B的第一份报告。蜡状芽孢杆菌菌株（DFE 4）具有芽孢杆菌霉素D生物合成基因。合成这些脂肽的细菌可以作为肽合成酶基因工程的天然遗传来源，用于生产新的肽。
Bacillus species are well known for their ability to control plant diseases through various mechanisms, including the production of secondary metabolites. Bacillus subtilis DFH08, an antagonist of Fusarium graminearum, and other Bacillus spp. that are antagonists of common fungal pathogens of canola were screened for peptide synthetase biosynthetic genes of fengycin and bacillomycin D. Specific polymerase chain reaction (PCR) primers identified B. subtilis strains DFH08 and 49 for the presence of the fenD gene of the fengycin operon. Bacillus cereus DFE4, Bacillus amyloliquefaciens strains DFE16 and BS6, and B. subtilis 49 were identified for the presence of the bamC gene of the bacillomycin D synthetase biosynthetic operon. Both fengycin and bacillomycin D were detected in the culture extract of strain Bs49, characterized through MALDI-TOF-MS (matrix-assisted laser desorption ionization - time of flight - mass spectrometry), and their antifungal activities demonstrated against F. graminearum and Sclerotinia sclerotiorum. This study designed and used specific PCR primers for the detection of potential fengycin- and bacillomycin D-producing bacteria antagonists and confirmed the molecular detection with the biochemical detection of the corresponding antibiotic produced. This is also the first report of a B. cereus strain (DFE4) to have bacillomycin D biosynthetic genes. Bacteria that synthesize these lipopeptides could act as natural genetic sources for genetic engineering of the peptide synthetases for production of novel peptides.