Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters
Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters
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DOI：
10.1111/j.1469-8137.2007.02331.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Versaw, Wayne K.
中科院分区：
文献类型：
--
作者：
Guo, B.;Jin, Y.;Versaw, Wayne K.
磷酸盐（Pi）在亚细胞区室之间的转运是代谢调节的核心。虽然一些参与控制细胞内分布的Pi的转运蛋白已被确定在植物中，其他预测从遗传，生物化学和bioinformatics study. Heteroimmune在酵母中的表达，在植物中的基因表达和定位被用来表征所有六个成员的拟南芥膜转运蛋白家族在这里指定为PHT 4。PHT 4蛋白与SLC 17/type I Pi转运蛋白（一组参与Pi、有机阴离子和氯离子转运的不同动物蛋白）具有相似性，所有PHT 4蛋白都以高度特异性介导酵母中的Pi转运。对PHT 4-GFP融合蛋白的生物信息学分析和定位结果表明，其中5个蛋白定位于质体被膜，6个蛋白定位于高尔基体; PHT 4基因在根和叶中均有表达，其中2个基因主要在叶中表达，1个基因主要在根中表达。这些表达模式，连同Pi的运输活动和亚细胞位置，表明PHT 4蛋白在胞质溶胶和叶绿体，异养质体和高尔基体之间的运输Pi的作用。
The transport of phosphate (Pi) between subcellular compartments is central to metabolic regulation. Although some of the transporters involved in controlling the intracellular distribution of Pi have been identified in plants, others are predicted from genetic, biochemical and bioinformatics studies.Heterologous expression in yeast, and gene expression and localization in plants were used to characterize all six members of an Arabidopsis thaliana membrane transporter family designated here as PHT4. PHT4 proteins share similarity with SLC17/type I Pi transporters, a diverse group of animal proteins involved in the transport of Pi, organic anions and chloride.All of the PHT4 proteins mediate Pi transport in yeast with high specificity. Bioinformatic analysis and localization of PHT4-GFP fusion proteins indicate that five of the proteins are targeted to the plastid envelope, and the sixth resides in the Golgi apparatus.PHT4 genes are expressed in both roots and leaves, although two of the genes are expressed predominantly in leaves and one mostly in roots. These expression patterns, together with Pi transport activities and subcellular locations, suggest roles for PHT4 proteins in the transport of Pi between the cytosol and chloroplasts, heterotrophic plastids and the Golgi apparatus.