Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis
Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis
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DOI：
10.1073/pnas.0408260101
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发表时间：
2004-12-28
影响因子：
11.1
通讯作者：
Dulbecco, R
中科院分区：
文献类型：
--
作者：
Zucchi, I;Mento, E;Dulbecco, R
乳腺癌的表达谱很难解释，当它们从组织中获得时，其中可能含有大比例的非癌细胞。为了避免这个问题，我们从原发性乳腺浸润性导管癌和转移性乳腺癌的腋窝淋巴结中显微镜下分离细胞，以获得纯的癌细胞群（约500个），并将其用于基因表达的系列分析。从两个群体的细胞产生的表达谱进行了比较与一个无病乳腺上皮细胞的配置文件。我们发现，所获得的表达谱不包括癌细胞，没有非上皮细胞的贡献。从总共分析的16，939个独特标签中，我们检测到559个基因表达的统计学显著变化;其中一些基因以前与乳腺癌无关。我们观察到，在两种癌症中，许多下调的基因是相同的，而上调的基因是完全不同的，这表明一组基因的下调可能是癌症形成的基本机制，而上调可能表征并可能控制个体癌症的演变状态。所获得的结果可能有助于表征乳腺癌的肿瘤过程。
Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (approximate to500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.