Nanochannel-Ion Channel Hybrid Device for Ultrasensitive Monitoring of Biomolecular Recognition Events
Nanochannel-Ion Channel Hybrid Device for Ultrasensitive Monitoring of Biomolecular Recognition Events
复制标题
用于生物分子识别事件超灵敏监测的纳米通道-离子通道混合装置
DOI：
10.1021/acs.analchem.8b05162
复制
发表时间：
2019
影响因子：
7.4
通讯作者：
Xia Xing-Hua
中科院分区：
文献类型：
--
作者：
Zhao Xiao-Ping;Zhou Yue;Zhang Qian-Wen;Yang Dong-Rui;Wang Chen;Xia Xing-Hua
我们提出了一种原位和无标记的方法来检测生物分子识别事件，使用纳米通道-离子通道混合装置集成了电化学检测器。首先将适配体固定在纳米通道-离子通道杂化物的外表面。由于不同的表面电荷密度和有效通道大小，它与溶液中靶凝血酶的结合极大地调节了器件的传质行为。通过电化学检测器，可以实时监测质量传递特性的变化，从而实现凝血酶适体识别的原位和无标记检测。溶液pH值对检测灵敏度有显著影响。在最佳pH条件下，凝血酶的检出限可低至0.22 fM，远低于大多数报道的工作。该装置为生物分子识别事件的监测提供了一个简单、超灵敏、无标记的平台，在探索生物分子在生命系统中的功能和反应机制方面具有很大的潜力。
We propose an in situ and label-free method for detection of biomolecular recognition events by use of a nanochannel–ion channel hybrid device integrated with an electrochemical detector. The aptamer is first immobilized on the outer surface of the nanochannel–ion channel hybrid. Its binding with target thrombin in solution considerably regulates the mass-transfer behavior of the device owing to the varied surface charge density and effective channel size. Via the electrochemical detector, the changed mass-transport property can be monitored in real time, which enables in situ and label-free detection of thrombin–aptamer recognition. The solution pH has a significant influence on detection sensitivity. Under optimal pH conditions, a detection limit as low as 0.22 fM thrombin can be achieved, which is much lower than most reported work. The present nanofluidic device provides a simple, ultrasensitive, and label-free platform for monitoring biomolecular recognition events, which would hold great potential in exploring the functions and reaction mechanisms of biomolecules in living systems.