Comparison of messenger RNA distribution for 60 proteins in fat cells vs the nonfat cells of human omental adipose tissue
Comparison of messenger RNA distribution for 60 proteins in fat cells vs the nonfat cells of human omental adipose tissue
复制标题
DOI：
10.1016/j.metabol.2008.02.019
复制
发表时间：
2008-07-01
影响因子：
9.8
通讯作者：
Madan, Atul K.
中科院分区：
文献类型：
--
作者：
Fain, John N.;Buehrer, Ben;Madan, Atul K.
在通过胶原酶消化获得的网膜脂肪组织的3个部分（脂肪细胞和包含胶原酶消化后剩余的组织的非事实细胞[基质]和基质血管细胞）中检查了60种蛋白质的信使RNA（mRNA）分布，所述网膜脂肪组织从接受减肥手术的病态肥胖妇女获得。与非脂肪细胞相比，脂肪细胞在视黄醇结合蛋白4、血管紧张素原、脂蛋白酶、谷胱甘肽过氧化物酶3、解偶联蛋白2、过氧化物酶体增殖物激活受体γ、细胞死亡诱导DFFA样效应子A、脂肪特异性蛋白27、11 β-羟基类固醇脱氢酶1、甘油通道水通道蛋白7、NADPH：醌氧化还原酶1、环腺苷一磷酸磷酸二酯酶3B、甘油醛-3-磷酸脱氢酶、胰岛素受体和淀粉样蛋白A1。脂肪细胞中还富集了至少26倍的参与脂解的蛋白质的mRNA，如脂肪敏感性脂肪酶、脂蛋白脂肪酶、脂肪组织甘油三酯脂肪酶和FAT/CD 36。mRNA在培养的前脂肪细胞中的相对分布也与来自人网膜脂肪组织的体外分化的脂肪细胞进行了比较。培养的前脂肪细胞的炎症蛋白的mRNA水平远低于网膜脂肪组织的非脂肪细胞。在非脂肪细胞中，参与炎症反应的蛋白质（如肿瘤坏死因子α、白细胞介素1 β、环氧合酶2、白细胞介素24、白细胞介素6和单核细胞趋化蛋白1）的mRNA以及骨桥蛋白、vaspin、内皮素、血管紧张素II受体1、丁酰胆碱酯酶、脂质运载蛋白2和纤溶酶原激活物抑制剂1的mRNA至少富集5倍。与分离的基质血管细胞相比，脂肪组织基质中的细胞在与炎症反应相关的蛋白质以及骨桥蛋白和内皮型一氧化氮合酶的mRNA中富集至少3倍。我们的结论是，炎症蛋白的mRNA主要存在于人类网膜脂肪组织的非脂肪细胞。
The messenger RNA (mRNA) distribution of 60 proteins was examined in the 3 fractions obtained by collagenase digestion (fat cells and the nonfact cells comprising the tissue remaining after collagenase digestion [matrix] and the stromovascular cells) of omental adipose tissue obtained from morbidly obese women undergoing bariatric surgery. Fat cells were enriched by at least 3-fold as compared with nonfat cells in the mRNAs for retinol binding protein 4, angiotensinogen, adipsin, glutathione peroxidase 3, uncoupling protein 2, peroxisome proliferator-activated receptor gamma, cell death-inducing DFFA-like effector A, fat-specific protein 27, 11 beta-hydroxysteroid dehydrogenase 1, glycerol channel aquaporin 7, NADPH:quinone oxidoreductase 1, cyclic adenosine monophosphate phosphodiesterase 3B, glyceraldehyde-3-phosphate dehydrogenase, insulin receptor, and amyloid A1. Fat cells were also enriched by at least 26-fold in the mRNAs for proteins involved in lipolysis such as hormone-sensitive lipase, lipoprotein lipase, adipose tissue triglyceride lipase, and FAT/CD36. The relative distribution of mRNAs in cultured preadipocytes was also compared with that of in vitro differentiated adipocytes derived from human omental adipose tissue. Cultured preadipocytes had far lower levels of the mRNAs for inflammatory proteins than the nonfat cells of omental adipose tissue. The nonfat cells were enriched by at least 5-fold in the mRNAs for proteins involved in the inflammatory response such as tumor necrosis factor alpha, interleukin 1 beta, cyclooxygenase 2, interleukin 24, interleukin 6, and monocyte chemoattractant protein 1 plus the mRNAs for osteopontin, vaspin, endothelin, angiotensin II receptor 1, butyrylcholinesterase, lipocalin 2, and plasminogen activator inhibitor 1. The cells in the adipose tissue matrix were enriched at least 3-fold as compared with the isolated stromovascular cells in the mRNAs for proteins related to the inflammatory response, as well as osteopontin and endothelial nitric oxide synthase. We conclude that the mRNAs for inflammatory proteins are primarily present in the nonfat cells of human omental adipose tissue.