Enhancement of Colorimetric Response of Enzymatic Reactions by Thermally Evaporated Plasmonic Thin Films: Application to Glial Fibrillary Acidic Protein.
Enhancement of Colorimetric Response of Enzymatic Reactions by Thermally Evaporated Plasmonic Thin Films: Application to Glial Fibrillary Acidic Protein.
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DOI：
10.1039/c4ay02505a
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发表时间：
2015-02-07
期刊：
影响因子：
--
通讯作者：
Aslan K
中科院分区：
文献类型：
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作者：
Abel B;Kabir TS;Odukoya B;Mohammed M;Aslan K
本文报道了热蒸发银、金、铜和镍薄膜在生物测定中对辣根过氧化物酶(HRP)和碱性磷酸酶(AP)的显色响应的增强。在这方面，采用了基于生物素-亲和素相互作用的模型生物测定。生物素基团和酶分别使用生物素化的连接物分子和亲和素引入到所有表面。在等离子体薄膜上进行的模型生物测定中，HRP的比色响应比对照样品(即没有等离子体薄膜)大4.4倍，其中在银薄膜上观察到的比色响应增强最大。AP在等离子体薄膜上的比色响应与在对照样品上观察到的相似，这归因于在生物测定步骤中表面酶的损失。固定在等离子体薄膜上的酶的程度被发现影响了模型生物测定的比色响应。这些发现使我们能够展示使用银膜来检测胶质纤维酸性蛋白(GFAP)，其中对GFAP的标准生物检测方法的比色响应比玻璃片上的生物检测方法提高了67%。
We report the enhancement of the colorimetric response of horseradish peroxidase (HRP) and alkaline phosphatase (AP) in bioassays by thermally evaporated silver, gold, copper and nickel thin films. In this regard, a model bioassay based on biotin-avidin interactions was employed. Biotin groups and enzymes were introduced to all surfaces using a biotinylated linker molecule and avidin, respectively. The colorimetric response of HRP in the model bioassay carried out on the plasmonic thin films were up to 4.4-fold larger as compared to control samples (i.e., no plasmonic thin films), where the largest enhancement of colorimetric response was observed on silver thin films. The colorimetric response of AP on plasmonic thin films was found to be similar to those observed on control samples, which was attributed to the loss of enzymes from the surface during the bioassay steps. The extent of enzymes immobilized on to plasmonic thin films was found to affect the colorimetric response of the model bioassay. These findings allowed us to demonstrate the use of silver thin films for the detection of glial fibrillary acidic protein (GFAP), where the colorimetric response of the standard bioassays for GFAP was enhanced up to 67% as compared to bioassays on glass slides.