Supported lipid membranes with designed geometry
Supported lipid membranes with designed geometry
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具有设计几何形状的支撑脂质膜
DOI：
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发表时间：
2021
期刊：
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通讯作者：
Daniela J. Kraft
中科院分区：
文献类型：
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作者：
Melissa Rinaldin;S. Haaf;Ernst J. Vegter;C. V. D. Wel;Piermarco Fonda;L. Giomi;Daniela J. Kraft
细胞和细胞内区室的膜曲率不断适应，使细胞能够执行从细胞分裂到信号传输的重要功能。由于膜的复杂性以及所涉及的时间短和长度尺度小，了解膜几何形状如何影响体内的这些过程具有挑战性。相比之下，具有工程曲率的体外模型膜为这项研究以及生物传感和生物计算的应用提供了一个多功能平台。然而，仍然缺乏制造具有规定曲率和高空间分辨率的脂质膜的一般途径。在这里，我们提出了一种克服这些挑战的策略，通过结合 3D 微打印和复制成型光刻来创建具有几乎任何几何形状和高空间分辨率的支架，从而实现具有设计形状的脂质膜。所得的支撑脂质膜是均匀的、流动的，并且可以形成化学上不同的脂质域。这些特征对于理解曲率相关的细胞过程以及开发活细胞和纳米结构的可编程生物界面至关重要。
The membrane curvature of cells and intracellular compartments continuously adapts to enable cells to perform vital functions, from cell division to signal trafficking. Understanding how membrane geometry affects these processes in vivo is challenging because of the membrane complexity as well as the short time and small length scales involved. By contrast, in vitro model membranes with engineered curvature provide a versatile platform for this investigation and applications to biosensing and biocomputing. However, a general route to the fabrication of lipid membranes with prescribed curvature and high spatial resolution is still missing. Here, we present a strategy that overcomes these challenges and achieve lipid membranes with designed shape by combining 3D micro-printing and replica-molding lithography to create scaffolds with virtually any geometry and high spatial resolution. The resulting supported lipid membranes are homogeneous, fluid, and can form chemically distinct lipid domains. These features are essential for understanding curvature-dependent cellular processes and developing programmable bio-interfaces for living cells and nanostructures.