Moderate doses of conjugated linoleic acid reduce fat gain, maintain insulin sensitivity without impairing inflammatory adipose tissue status in mice fed a high-fat diet.
Moderate doses of conjugated linoleic acid reduce fat gain, maintain insulin sensitivity without impairing inflammatory adipose tissue status in mice fed a high-fat diet.
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DOI：
10.1186/1743-7075-7-5
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发表时间：
2010-01-20
影响因子：
4.5
通讯作者：
Serra F
中科院分区：
文献类型：
--
作者：
Parra P;Palou A;Serra F
用对身体成分有潜在好处的营养素丰富饮食是对抗肥胖的一种策略。共轭亚油酸(CLA)因其对身体成分和炎症过程的有益影响而成为一个有趣的候选物质，因为肥胖的促进和损害与脂肪组织的低级别炎症状态密切相关。此前，我们报道了中等剂量的CLA混合物对喂食标准脂肪饮食的小鼠的身体成分和脂肪组织炎症状态的有利影响。在目前的研究中，我们评估了CLA混合物(顺-9，反式-11和反式-10，顺式-12，50：50)在高脂饮食小鼠中的潜在益处。在研究的前30天，测定两个剂量：0.15g(CLA1)和0.5g CLA/kg体重(CLA2)，然后动物在接下来的35天里接受双倍剂量的CLA。最低剂量(CLA1)对体成分、血浆参数和基因表达的影响较小。然而，CLA2明显减少了脂肪积累，同时降低了瘦素、脂联素和非酯化脂肪酸(NEFA)的血浆浓度。尽管空腹血糖和胰岛素血浆浓度略有增加，但胰岛素敏感性仍保持不变。对脂肪细胞和间质血管部分(SVF)基因表达的研究表明，CLA可能减少脂肪组织中巨噬细胞的渗透或诱导促炎细胞因子的表达。总而言之，使用中等剂量的两种主要CLA异构体的等摩尔混合物，可以减少体内脂肪含量，改善血脂状况，保持胰岛素敏感性(尽管存在中等程度的高胰岛素血症)，而不会促进高脂饮食小鼠脂肪组织中的炎症标记物。
The enrichment of diet with nutrients with potential benefits on body composition is a strategy to combat obesity. Conjugated linoleic acid (CLA) due its beneficial effects on body composition and inflammatory processes becomes an interesting candidate, since the promotion and impairment of obesity is closely linked to a low-grade inflammation state of adipose tissue. Previously we reported the favourable effects of moderate doses of CLA mixture on body composition and inflammatory status of adipose tissue in mice fed a standard-fat diet. In the present study we assessed the potential beneficial effects of CLA mixture (cis-9, trans-11 and trans-10, cis-12, 50:50) in mice fed a high-fat diet. Two doses were assayed: 0.15 g (CLA1) and 0.5 g CLA/kg body weight (CLA2) for the first 30 days of the study and then animals received a double amount for another 35 days. The lowest dose (CLA1) had minor effects on body composition, plasma parameters and gene expression. However, a clear reduction in fat accumulation was achieved by CLA2, accompanied by a reduction in leptin, adiponectin and non-esterified fatty acids (NEFA) plasma concentrations. Insulin sensitivity was maintained despite a slight increase in fasting glucose and insulin plasma concentrations. The study of gene expression both in adipocytes and in the stromal vascular fraction (SVF) suggested that CLA may reduce either the infiltration of macrophages in adipose tissue or the induction of expression of pro-inflammatory cytokines. In conclusion, the use of moderate doses of an equimolar mix of the two main CLA isomers reduces body fat content, improves plasma lipid profile, maintains insulin sensitivity (despite a moderate degree of hyperinsulinaemia) without the promotion of inflammatory markers in adipose tissue of mice fed a high-fat diet.