Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma
Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma
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DOI：
10.1073/pnas.1004401107
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发表时间：
2010-09-14
影响因子：
11.1
通讯作者：
Pieler, Tomas
中科院分区：
文献类型：
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作者：
Koebernick, Katja;Loeber, Jana;Pieler, Tomas
未来生殖系的分离决定了动物发育过程中细胞命运的关键。在非洲爪蟾中，生殖细胞是由植物定位的母体决定因素遗传指定的，包括一组特定的mrna。在这里，我们发现爪蟾死端（XDE）的植物定位元件（LE）和其他几种种系特异性的植物定位转录物介导了爪蟾胚胎中微注射报告mRNA的生殖细胞特异性稳定和体细胞清除。XDE-LE中对体细胞RNA清除至关重要的部分与斑马鱼的nanos1具有同源性，并且似乎是Xenopus miR-18清除体细胞mRNA的靶点。爪蟾elr型植物定位复合物蛋白可减轻微注射XDE- le和内源性XDE mRNA对体细胞RNA的清除。ElrB1与爪蟾死端蛋白协同稳定XDE-LE mRNA。综上所述，我们的发现揭示了植物mRNA定位和种系mRNA免受体细胞清除保护的功能联系。
Segregation of the future germline defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.