In vitro activity of caspofungin against Candida albicans biofilms
In vitro activity of caspofungin against Candida albicans biofilms
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DOI：
10.1128/aac.46.11.3591-3596.2002
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发表时间：
2002-11-01
影响因子：
4.9
通讯作者：
López-Ribot, JL
中科院分区：
文献类型：
--
作者：
Bachmann, SP;VandeWalle, K;López-Ribot, JL
念珠菌病的大多数表现与生物或无生命表面上的生物膜形成有关。白色念珠菌生物膜对常规抗真菌治疗无效。在这里，我们报告了卡泊芬净，一种新的半合成棘白菌素，对C。白色念珠菌生物膜卡泊芬净对无柄C.白念珠菌细胞内的生物膜，与MIC的50%的固着细胞被抑制以及药物的治疗范围内。扫描电子显微镜和激光共聚焦扫描显微镜观察卡泊芬净对C。结果表明，卡泊芬净影响白念珠菌生物膜内细胞的形态和代谢状态。卡泊芬净对生物材料的涂层对随后的生物膜发展有抑制作用。白色念珠菌。这些结果共同表明卡泊芬净对C.白念珠菌生物膜的体外研究和生物膜相关感染的治疗值得进一步研究。
Most manifestations of candidiasis are associated with biofilm formation on biological or inanimate surfaces. Candida albicans biofilms are recalcitrant to treatment with conventional antifungal therapies. Here we report on the activity of caspofungin, a new semisynthetic echinocandin, against C. albicans biofilms. Caspofungin displayed potent in vitro activity against sessile C. albicans cells within biofilms, with MICs at which 50% of the sessile cells were inhibited well within the drug's therapeutic range. Scanning electron microscopy and confocal scanning laser microscopy were used to visualize the effects of caspofungin on preformed C. albicans biofilms, and the results indicated that caspofungin affected the cellular morphology and the metabolic status of cells within the biofilms. The coating of biomaterials with caspofungin had an inhibitory effect on subsequent biofilm development by C. albicans. Together these findings indicate that caspofungin displays potent activity against C. albicans biofilms in vitro and merits further investigation for the treatment of biofilm-associated infections.