Hypertrophic chondrocytes undergo further differentiation to osteoblast‐like cells and participate in the initial bone formation in developing chick embryo
Hypertrophic chondrocytes undergo further differentiation to osteoblast‐like cells and participate in the initial bone formation in developing chick embryo
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肥大软骨细胞进一步分化为成骨细胞样细胞，并参与发育中鸡胚的初始骨形成
DOI：
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发表时间：
1994
影响因子：
6.2
通讯作者：
R. Cancedda
中科院分区：
文献类型：
--
作者：
M. Galotto;G. Campanile;Grazia Robino;F. Cancedda;P. Bianco;R. Cancedda
肥大软骨细胞向成骨细胞样表型的分化发生在鸡胚胎胫骨早期或未来骨膜下的肥大软骨和血管管周围的软骨中。C前肽的免疫定位表明增生性软骨细胞可以合成I型胶原。酶细胞化学表明，在体内，进一步分化的肥大软骨细胞在初始矿物质沉积时表达碱性磷酸酶。通过BrdU标记获得了肥大软骨细胞可能恢复增殖的证据。分离并鉴定了一种识别增生性软骨细胞膜蛋白的单克隆抗体（LA5）。LA5抗体除了染色被II型和X型胶原染色基质包围的增生性软骨细胞外，还染色软骨/骨领界面处的细长软骨细胞以及第一层骨和类骨基质中的细胞。
Differentiation of hypertrophic chondrocytes to an osteoblast‐like phenotype occurs in vivo in the hypertrophic cartilage of chick embryo tibiae underneath early or prospective periosteum and in cartilage around vascular canals. Synthesis of type I collagen by hypertrophic chondrocytes was shown by immunolocalization of the C propeptide. By enzyme cytochemistry it was instead shown that, in vivo, further differentiating hypertrophic chondrocytes express alkaline phosphatase at the time of initial mineral deposition. Evidence that hypertrophic chondrocytes may resume proliferation was obtained by BrdU labeling. A monoclonal antibody (LA5) was isolated and characterized that recognizes a hypertrophic chondrocyte membrane protein. In addition to staining hypertrophic chondrocytes surrounded by a type II and type X collagen‐stainable matrix, the LA5 antibodies also stained elongated chondrocytes at the cartilage/bone collar interface and cells incorporated in the first layer of bone and osteoid matrix.
死亡发育不良中的异常骨化。
影响因子：
4.1
作者：
Horton,WA;Hood,OJ;Machado,MA;Ahmed,S;Griffey,ES
通讯作者：
Griffey,ES
在幼鼠软骨四肢的初始成骨过程中，可能从肥大的软骨细胞中招募成骨细胞前体细胞。
DOI：
10.1016/s0934-8832(89)80001-0
发表时间：
1989
期刊：
Matrix (Stuttgart, Germany)
影响因子：
--
作者：
Franzen,A;Oldberg,A;Solursh,M
通讯作者：
Solursh,M