Proper placement of the Escherichia coli division site requires two functions that are associated with different domains of the MinE protein.
Proper placement of the Escherichia coli division site requires two functions that are associated with different domains of the MinE protein.
复制标题
大肠杆菌分裂位点的正确放置需要与 MinE 蛋白的不同结构域相关的两个功能。
DOI：
10.1073/pnas.92.10.4313
复制
发表时间：
1995
影响因子：
11.1
通讯作者：
Rothfield,LI
中科院分区：
文献类型：
--
作者：
Zhao,CR;deBoer,PA;Rothfield,LI
大肠埃希氏菌分离隔膜的正确放置需要MINE蛋白。Mine通过赋予Minc和Mind基因编码的分裂抑制因子拓扑特异性来实现这一点。结果，分裂抑制物阻止了存在于细胞两极的潜在分裂部位的分裂，但允许在中细胞的正常分裂部位分离。在这篇文章中，我们定义了这一效应所需的两个我的功能，并提出了88个氨基酸的MINE蛋白中不同的结构域负责这两个功能的证据。第一个结构域负责Mine中和MinCD分裂抑制物活性的能力，位于蛋白质N末端附近的一个小区域。第二个结构域是Mine功能的拓扑特异性所必需的，它位于蛋白质的更远端区域，即使与负责抑制分裂抑制活性的结构域分离时，也会影响分裂间隔放置的位置特异性。
The proper placement of the Escherichia coli division septum requires the MinE protein. MinE accomplishes this by imparting topological specificity to a division inhibitor coded by the minC and minD genes. As a result, the division inhibitor prevents septation at potential division sites that exist at the cell poles but permits septation at the normal division site at midcell. In this paper, we define two functions of MinE that are required for this effect and present evidence that different domains within the 88-amino acid MinE protein are responsible for each of these two functions. The first domain, responsible for the ability of MinE to counteract the activity of the MinCD division inhibitor, is located in a small region near the N terminus of the protein. The second domain, required for the topological specificity of MinE function, is located in the more distal region of the protein and affects the site specificity of placement of the division septum even when separated from the domain responsible for suppression of the activity of the division inhibitor.