Manganese-Enhanced MRI of the DBA/2J Mouse Model of Hereditary Glaucoma
Manganese-Enhanced MRI of the DBA/2J Mouse Model of Hereditary Glaucoma
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DOI：
10.1167/iovs.08-2205
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Berkowitz, Bruce A.
中科院分区：
文献类型：
--
作者：
Calkins, David J.;Horner, Philip J.;Berkowitz, Bruce A.
目的.为验证锰增强磁共振成像（MEMRI）是一种敏感的测量实验性色素性青光眼年龄相关性眼部变化的方法。使用MEMRI研究了四组光适应小鼠：年轻（2-3个月）、C57 BL/6（阴性对照）和DBA/2 J小鼠以及老年（10-11个月）C57 BL/6和DBA/2 J小鼠。在所有小鼠中，根据MEMRI数据评估眼周、视神经乳头宽度、虹膜角膜角、睫状体面积、总视网膜厚度和内部视网膜厚度以及视网膜离子调节的替代物（视网膜内锰摄取）并进行比较。从MEMRI评估的眼睛收获的视神经获得轴突计数。随着年龄的增长，C57 BL/6和DBA/2 J小鼠的眼周长、视网膜厚度、虹膜角膜角、睫状体面积和视神经乳头宽度的差异和显著变化很容易从MEMRI数据中测量（P < 0.05）。在C57 BL/6小鼠中，仅视网膜内层厚度和周长相关。在DBA/2 J小鼠中，眼周与视网膜总厚度和内部厚度、睫状体面积、视神经乳头宽度和虹膜角膜角相关。青年和老年小鼠的比较显示，老年DBA/2 J小鼠视网膜内锰摄取低于正常（P < 0.05），而老年C57 BL/6小鼠没有。锰的摄取与眼周无相关性。视神经轴突密度与MEMRI测量的视神经乳头宽度相关（P < 0.05）。这些研究在遗传性青光眼的常见动物模型中提供了与年龄相关的非侵入性MEMRI可检测变化的基线，这可能有助于治疗成功的纵向评价。（Invest Ophthalmol维斯科学。2008; 49：5083-5088）DOI：10.1167/iovs.082205
PURPOSE. To test the hypothesis that manganese-enhanced magnetic resonance imaging (MEMRI) is a sensitive approach for measuring of age-related ocular changes in experimental pigmentary glaucoma.METHODS. Four groups of light-adapted mice were studied using MEMRI: young (2-3 months), C57BL/6 (negative controls), and DBA/2J mice and aged (10-11 months) C57BL/6 and DBA/2J mice. In all mice, eye perimeter, optic nerve head width, iridocorneal angle, ciliary body area, and total and inner retinal thickness, and a surrogate of retinal ion regulation (intraretinal uptake of manganese) were assessed from MEMRI data and compared. Axon counts were obtained from optic nerves harvested from MEMRI-assessed eyes.RESULTS. As the C57BL/6 and DBA/2J mice aged, differential and significant changes in ocular perimeter, retinal thickness, iridocorneal angle, ciliary body area, and optic nerve head width were readily measured from MEMRI data (P < 0.05). In C57BL/6 mice, only inner retinal thickness and perimeter were correlated. In DBA/2J mice, ocular perimeter was correlated with total and inner retinal thickness, ciliary body area, optic nerve head width, and iridocorneal angle. Comparison of young and aged mice revealed a subnormal intraretinal manganese uptake (P < 0.05) in aged DBA/2J mice, but not in aged C57BL/6 mice. Manganese uptake did not correlate with the ocular perimeter. Axon density in the optic nerve correlated with MEMRI-measured optic nerve head width (P < 0.05).CONCLUSIONS. These studies provide a baseline of noninvasive MEMRI-detectable changes associated with age in a common animal model of hereditary glaucoma that may be useful in the longitudinal evaluation of therapeutic success. (Invest Ophthalmol Vis Sci. 2008; 49: 5083-5088) DOI: 10.1167/iovs.082205