Circulating CD31+ annexin V+ apoptotic microparticles correlate with coronary endothelial function in patients with coronary artery disease
Circulating CD31+ annexin V+ apoptotic microparticles correlate with coronary endothelial function in patients with coronary artery disease
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DOI：
10.1161/01.atv.0000191634.13057.15
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发表时间：
2006-01-01
影响因子：
8.7
通讯作者：
Nickenig, G
中科院分区：
文献类型：
--
作者：
Werner, N;Wassmann, S;Nickenig, G
目的-内皮功能障碍预测心血管风险患者的发病率和死亡率。内皮功能可能是决定性的影响内皮细胞凋亡的程度。方法和结果-为了测试这一假设在人类，内皮依赖性血管舒张进行了侵入性评估，在50例冠状动脉疾病（CAD）冠状动脉内乙酰胆碱输注定量冠状动脉造影术。流式细胞术通过定量外周血中循环CD 31（+）/膜联蛋白V+凋亡微粒来评估内皮细胞凋亡。凋亡微粒计数增加与冠状动脉内皮功能受损呈正相关。多变量分析显示，增加凋亡微粒计数预测严重的内皮功能障碍独立于经典的危险因素，如高血压，高胆固醇血症，吸烟，糖尿病，年龄，或sex.Conclusions -在CAD患者中，内皮依赖性血管舒张密切依赖于内皮细胞凋亡的程度，这是很容易测量的循环CD 31（+）/膜联蛋白V+凋亡微粒。这些发现可能为风险评估提供新的选择，并可能对未来的CAD治疗策略产生影响。
Objective - Endothelial dysfunction predicts morbidity and mortality in patients at cardiovascular risk. Endothelial function may be decisively influenced by the degree of endothelial cell apoptosis.Methods and Results - To test this hypothesis in humans, endothelial-dependent vasodilatation was invasively assessed in 50 patients with coronary artery disease (CAD) by quantitative coronary angiography during intracoronary acetylcholine infusion. Flow cytometry was used to assess endothelial cell apoptosis by quantification of circulating CD31(+)/annexin V+ apoptotic microparticles in peripheral blood. Increased apoptotic microparticle counts positively correlated with impairment of coronary endothelial function. Multivariate analysis revealed that increased apoptotic microparticle counts predict severe endothelial dysfunction independent of classical risk factors, such as hypertension, hypercholesterolemia, smoking, diabetes, age, or sex.Conclusions - In patients with CAD, endothelial-dependent vasodilatation closely relies on the degree of endothelial cell apoptosis, which is readily measurable by circulating CD31(+)/annexin V+ apoptotic microparticles. These findings possibly provide new options for risk assessment and may have implications for future treatment strategies of CAD.