HISTOLOGICAL EVIDENCE SUPPORTING INFERIOR OLIVE AS MAJOR SOURCE OF CEREBELLAR CLIMBING FIBERS IN RAT
HISTOLOGICAL EVIDENCE SUPPORTING INFERIOR OLIVE AS MAJOR SOURCE OF CEREBELLAR CLIMBING FIBERS IN RAT
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DOI：
10.1016/0006-8993(74)90628-3
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
DESCLIN, JC
中科院分区：
文献类型：
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作者：
DESCLIN, JC
通过 3-乙酰吡啶中毒，大鼠的双侧下橄榄被完全破坏。通过一种新的银方法，选择性地浸渍退化的神经细胞突起和核周，在 35 只接受治疗的大鼠的小脑皮层中研究了橄榄小脑纤维末端的主动继发性变性。早在处理后 12 小时，就在分子层和颗粒层中观察到退化的攀缘纤维。存活 48 小时后，不再检测到退化的攀爬纤维。本研究进行了以下观察。 (1) 所有橄榄小脑纤维均以小脑攀爬纤维结束；劣质橄榄不是苔藓纤维的来源。 (2)攀爬纤维分支频繁，主要分布在神经节下丛。因此，得出的结论是，单个橄榄神经元可以为多个浦肯野细胞提供攀爬纤维。 (3)由于下橄榄体完全破坏，观察到所有攀缘纤维在小脑皮质的大面积上退化。在作为整体的实验材料中，攀爬纤维的大量退化发生在分子层的整个范围内。因此，可以得出结论，下橄榄是小脑攀爬纤维的主要且可能是唯一重要的来源。
Complete bilateral destruction of the inferior olive was achieved in rats by 3-acetylpyridine intoxication. Active secondary degeneration of olivo-cerebellar fiber terminals was studied in the cerebellar cortex of 35 treated rats by means of a new silver method which selectively impregnated degenerating nerve cell processes and perikarya. Degenerating climbing fibers were observed in both the molecular and the granular layers as early as 12 h after the treatment. Degenerating climbing fibers were no longer detectable beyond 48 h of survival. The following observations were made in this study. (1) All olivo-cerebellar fibers end as climbing fibers in the cerebellum; the inferior olive is not a source of mossy fibers. (2) Climbing fibers branch frequently, mainly in the infra-ganglionic plexus. Hence, it is concluded that a single olivary neuron can provide several Purkinje cells with climbing fibers. (3) As a consequence of complete inferior olive destruction, all climbing fibers were observed to degenerate over large areas of the cerebellar cortex. In the experimental material taken as a whole, massive degeneration of climbing fibers occurred over the entire extent of the molecular layer. Therefore, it is concluded that the inferior olive is the major and probably the sole significant source of cerebellar climbing fibers.