Effect of plasmid-mediated stable interferon-γ expression on proliferation and cell death in the SKOV-3 human ovarian cancer cell line
Effect of plasmid-mediated stable interferon-γ expression on proliferation and cell death in the SKOV-3 human ovarian cancer cell line
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DOI：
10.3109/08923973.2010.543685
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发表时间：
2011-09-01
影响因子：
3.3
通讯作者：
Guan, Yongmei
中科院分区：
文献类型：
--
作者：
Lv, Huiming;Zhang, Huiya;Guan, Yongmei
前言：干扰素-γ在肿瘤中的高表达被认为是一个良好的预后指标。以往的研究表明，卵巢癌细胞持续暴露于干扰素-γ可导致显著的生长抑制和细胞凋亡。本研究的目的是评价载体介导的干扰素-γ稳定表达对人卵巢癌细胞系SKOV-3的影响。逆转录-聚合酶链式反应(RT-PCR)检测干扰素-γ(IFN-Gamma)基因表达，酶联免疫吸附试验(EL ISA)检测干扰素-γ蛋白表达。用四甲基偶氮唑蓝比色法和Hoechst 33258染色分别检测细胞增殖和细胞死亡，并与未转染组和空载体转染组进行比较。与未转染组或空载体转染组相比，它们的细胞增殖显著降低(P<；0.05)。结论：稳定表达的干扰素-γ可显著抑制卵巢癌细胞的增殖，有望用于临床治疗卵巢癌。
Introduction: High interferon-gamma (IFN-gamma) expression in tumors has been reported to be a favorable prognostic marker. Continuous exposure of ovarian cancer cells to IFN-gamma was previously shown to result in significant growth inhibition and apoptosis. Our goal in this study was to evaluate the effect of plasmid-mediated stable IFN-gamma expression on the SKOV-3 human ovarian carcinoma cell line.Methods: SKOV-3 cells were stably transfected with the pEGFP-IFN-gamma plasmid. IFN-gamma mRNA was detected by RT-PCR and IFN-gamma protein expression was measured by ELISA. Proliferation and cell death in transfected SKOV-3 cells were measured by methyl-thiazolyl tetrazolium (MTT) assay and Hoechst 33258 staining, respectively and compared with untransfected and empty vector-transfected cells.Results: pEGFP-IFN-gamma SKOV-3 cells efficiently expressed and secreted IFN-gamma. They exhibited significantly decreased cellular proliferation when compared with control untransfected or empty vector-transfected cells (P < 0.05). The mode of cell death was primarily apoptosis.Conclusions: Stable expression of IFN-gamma significantly inhibits the proliferation of ovarian carcinoma cells and has the potential to be used in clinical applications to treat ovarian carcinoma in the future.