Comparative Analysis of Mouse Decidualization Models at the Molecular Level
Comparative Analysis of Mouse Decidualization Models at the Molecular Level
复制标题
小鼠蜕膜化模型的分子水平比较分析
DOI：
10.3390/genes11080935
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发表时间：
2020-08
期刊：
影响因子：
--
通讯作者：
Liu Ji-Long
中科院分区：
文献类型：
--
作者：
Wang Chong;Zhao Miao;Zhang Wen-Qian;Huang Ming-Yu;Zhu Can;He Jia-Peng;Liu Ji-Long
小鼠被广泛用于研究蜕膜化，并且存在三种成熟的小鼠蜕膜化模型，即自然妊娠蜕膜化（NPD）、人工蜕膜化（AD）和体外蜕膜化（IVD）。然而，这些模型在分子水平上的相似性和差异程度在很大程度上仍然未知。在这里，我们使用RNA-seq方法进行了比较分析。在被认为是小鼠蜕膜化的金标准的NPD模型中，我们共发现了5277个差异表达基因，其中3158个基因表达上调，2119个基因表达下调。在AD模型中共鉴定出4294个差异表达基因，其中上调基因1127个，下调基因3167个。与NPD相比，1977个基因一致表达，而只有217个基因不一致表达，表明AD是小鼠蜕膜化的可靠模型。在IVD模型中，RNA-seq分析显示513个基因上调，988个基因下调。与NPD相比，310个基因一致表达，而456个基因不一致表达。此外，尽管蜕膜化标志物Prl 8a 2（催乳素家族8亚家族a成员2）在IVD模型中上调，但广泛使用的标志物Alpl（碱性磷酸酶肝/骨/肾）在IVD模型中下调。因此，我们建议IVD模型应进行优化，以在转录组水平上模拟NPD。我们的研究有助于增加有关小鼠蜕膜化模型的知识。
The mouse is widely used to study decidualization and there are three well-established mouse models of decidualization, namely natural pregnancy decidualization (NPD), artificial decidualization (AD), and in vitro decidualization (IVD). However, the extent of similarity and difference between these models at the molecular level remains largely unknown. Here, we performed a comparative analysis using the RNA-seq approach. In the NPD model, which is thought to be the golden standard of mouse decidualization, we found a total of 5277 differentially expressed genes, with 3158 genes being up-regulated and 2119 genes being down-regulated. A total of 4294 differentially expressed genes were identified in the AD model: 1127 up-regulated genes and 3167 down-regulated genes. In comparison to NPD, 1977 genes were consistently expressed, whereas only 217 genes were inconsistently expressed, indicating that AD is a reliable model for mouse decidualization. In the IVD model, RNA-seq analysis revealed that 513 genes were up-regulated and 988 genes were down-regulated. Compared to NPD, 310 genes were consistently expressed, whereas 456 genes were inconsistently expressed. Moreover, although the decidualization marker Prl8a2 (prolactin family 8 subfamily a member 2) was up-regulated, the widely-used marker Alpl (alkaline phosphatase liver/bone/kidney) was down-regulated in the IVD model. Therefore, we suggest that the IVD model should be optimized to mimic NPD at the transcriptomic level. Our study contributes to an increase in the knowledge about mouse models of decidualization.
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影响因子：
3.5
作者：
Chakraborty, I;Das, SK;Dey, SK
通讯作者：
Dey, SK
影响因子：
4.5
作者：
Large MJ;Wetendorf M;Lanz RB;Hartig SM;Creighton CJ;Mancini MA;Kovanci E;Lee KF;Threadgill DW;Lydon JP;Jeong JW;DeMayo FJ
通讯作者：
DeMayo FJ
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
DOI：
10.1073/pnas.1508056112
发表时间：
2015-11
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Shannon D. Whirledge;R. Oakley;P. Myers;J. Lydon;F. DeMayo;J. Cidlowski
通讯作者：
Shannon D. Whirledge;R. Oakley;P. Myers;J. Lydon;F. DeMayo;J. Cidlowski
影响因子：
4.8
作者：
Li, Quanxi;Kannan, Athilakshmi;Bagchi, Indrani C.
通讯作者：
Bagchi, Indrani C.