A parathyroid hormone-like protein from cultured human keratinocytes.
A parathyroid hormone-like protein from cultured human keratinocytes.
复制标题
来自培养的人角质形成细胞的甲状旁腺激素样蛋白质。
DOI：
10.1126/science.2417317
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Stewart,AF
中科院分区：
文献类型：
--
作者：
MerendinoJr,JJ;Insogna,KL;Milstone,LM;Broadus,AE;Stewart,AF
甲状旁腺素样因子已在与恶性体液性高钙血症相关的肿瘤提取物中发现，其中许多是鳞状上皮起源的。培养的，非恶性的人角质形成细胞进行了检查，生产类似的因素。角化细胞条件培养基刺激产生环磷酸腺苷在克隆衍生的大鼠骨肉瘤细胞对甲状旁腺激素敏感。牛[Nle 8，18，Tyr 34]PTH-（3-34）NH 2是甲状旁腺激素的竞争性抑制剂，可阻断角质形成细胞条件培养液刺激的腺苷酸环化酶的产生，但甲状旁腺激素抗血清对腺苷酸环化酶活性无影响。角化细胞条件培养基的活性成分的分子量超过天然甲状旁腺激素的分子量。与恶性体液性高钙血症相关的甲状旁腺激素受体激动剂也具有这些特征，这表明正常人角质形成细胞可能产生与恶性体液性高钙血症相关的恶性肿瘤产生的因子相关的因子。
Parathyroid hormone-like factors have been found in extracts of tumors associated with humoral hypercalcemia of malignancy, many of which are of squamous epithelial origin. Cultured, nonmalignant human keratinocytes were examined for the production of similar factors. Keratinocyte-conditioned medium from ten cultures stimulated the production of cyclic adenosine monophosphate in clonally derived rat osteosarcoma cells sensitive to parathyroid hormone. Bovine [Nle8,18, Tyr34]PTH-(3-34)NH2, a competitive inhibitor of parathyroid hormone, stopped the adenylate cyclase production stimulated by keratinocyte-conditioned medium, but antisera to parathyroid hormone had no effect on such adenylate cyclase activity. The active component of keratinocyte-conditioned medium has a molecular weight exceeding that of native parathyroid hormone. These characteristics are shared by the parathyroid hormone receptor agonists associated with humoral hypercalcemia of malignancy, which suggests that normal human keratinocytes may produce a factor related to that produced by malignant tumors associated with humoral hypercalcemia of malignancy.