Identification of two novel genes specifically expressed in the D-group neurons of the terrestrial snail CNS
Identification of two novel genes specifically expressed in the D-group neurons of the terrestrial snail CNS
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DOI：
10.1007/bf02336661
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发表时间：
1996-06-01
影响因子：
--
通讯作者：
Belyavsky, AV
中科院分区：
文献类型：
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作者：
Bogdanov, YD;Balaban, PM;Belyavsky, AV
搜索在蜗牛顶神经节的巨型中间神经元中特异表达的基因，其中包括两个基因，命名为HDS1和HDS2。根据Northern杂交和整体原位杂交的数据，这两个基因都是神经特异性的，几乎只在位于右侧顶神经节的肽能D组神经元中表达(Sakharov，1974)。在HDS1和HDS2探针与几个螺科超家族相关物种的中枢神经系统的正弦杂交中，所有病例都发现了相似的同源神经元群。对HDS1和HDS2基因的近全长cDNA拷贝进行测序发现，HDS1和HDS2的开放阅读框分别为107和102个氨基酸。这两种推测的蛋白质都含有一个疏水的前导肽和可能的呋喃类和PC样内肽酶的识别位点。预测的HDS1和HDS2蛋白的氨基酸序列相互之间以及与淡水蜗牛淡黄色细胞表达的LYCP前激素原有一定的同源性。这些结果证实了一个较早的假说，即螺旋家族的D-群和Lymna ea stagnaris的淡黄色细胞代表同源神经元群。我们的数据表明，HDS1和HDS2基因编码分泌分子的前体，很可能是神经肽或神经激素。
A search for genes specifically expressed in the giant interneurons of parietal ganglia of the snail Helix lucorum yielded, among others, two genes named HDS1 and HDS2. According to data obtained by Northern hybridization and whole-mount in situ hybridization, both genes are neurospecific and expressed almost exclusively in the peptidergic D-group neurons (Sakharov, 1974) located in the right parietal ganglion. In sine hybridization of the HDS1 and HDS2 probes with CNS of several related species of the Helicoidea superfamily identified in all cases similarly located homologous groups of neurons. Sequencing of the near full-length cDNA copies of the HDS1 and HDS2 genes revealed open reading frames 107 and 102 amino acids long for HDS1 and HDS2, respectively. Both putative proteins contain a hydrophobic leader peptide and putative recognition sites for furin-like and PC-like endopeptidases. Predicted amino acid sequences of the HDS1 and HDS2 proteins were found to be moderately homologous to each other, as well as to the LYCP preprohormone expressed by the light yellow cells of the freshwater snail Lymnaea stagnalis. These results confirm an earlier hypothesis that the D-group of the Helix family and the light yellow cells of Lymnaea stagnalis represent homologous neuronal groups. Our data suggest that the HDS1 and HDS2 genes encode precursors of secreted molecules, most likely neuropeptides or neurohormones.