CD36 involvement in the olfactory perception of oleic aldehyde, an odour-active volatile compound, in mice
CD36 involvement in the olfactory perception of oleic aldehyde, an odour-active volatile compound, in mice
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DOI：
10.2220/biomedres.38.207
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发表时间：
2017-01-01
影响因子：
1.2
通讯作者：
Inoue, Kazuo
中科院分区：
文献类型：
--
作者：
Lee, Shinhye;Tsuzuki, Satoshi;Inoue, Kazuo
分化簇36（CD36）是一种广泛表达的跨膜受体，具有多个配体。已发现其大量存在于小鼠的嗅上皮表面，并被假定在哺乳动物的嗅觉中起作用。然而，有没有行为学分析的哺乳动物的行为显示CD36参与嗅觉感知的一个独特的气味活性挥发性化合物。在这项研究中，我们的目的是评估哺乳动物是否感知到油酸醛，一种气味活性挥发物，作为一个潜在的CD36配体，如果是这样，是否CD36参与以下测量使用CD36基因敲除小鼠和他们的野生型同窝仔的传感。在两瓶选择试验中，野生型小鼠，而不是CD36敲除小鼠，区分含有油酸醛的蔗糖溶液和单独的蔗糖溶液。为了评估嗅觉系统在油醛感知中的重要性，我们进行了一项探索试验，其中动物可以主要依靠测试挥发物的气味进行识别。我们发现，野生型，但不是CD36敲除小鼠，知道的化合物。我们的研究结果提供了行为证据，CD36在鼻腔中特定气味活性挥发性化合物的感知中发挥作用。
Cluster of differentiation 36 (CD36) is a broadly expressed transmembrane receptor that has multiple ligands. It has been found to occur abundantly on the surface of the olfactory epithelium in mice and postulated to play a role in mammalian olfaction. However, there have been no ethological analyses of the mammalian behaviour showing CD36 involvement in the olfactory perception of a distinct odour-active volatile compound. In this study, we aimed to assess whether mammals perceive oleic aldehyde, an odour-active volatile that serves as a potential CD36 ligand, and if so, whether CD36 is involved in the sensing by following measurements using CD36-knockout mice and their wild-type littermates. In a two-bottle choice test, wild-type mice, but not CD36-knockout mice, discriminated a sucrose solution containing oleic aldehyde from the sucrose solution alone. To assess the importance of the olfactory system in the oleic aldehyde perception, we conducted an exploration test where the animals could rely primarily on the odour of test volatiles for recognition. We found that the wild-type, but not CD36-knockout mice, were aware of the compound. Our results provide behavioural evidence that CD36 plays a role in the perception of specific odour-active volatile compounds in the nasal cavity.