HPLC and HPCE analysis of microcystins RR, LR and YR present in cyanobacteria and water by using immunoaffinity extraction
HPLC and HPCE analysis of microcystins RR, LR and YR present in cyanobacteria and water by using immunoaffinity extraction
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DOI：
10.1016/s0039-9140(02)00610-0
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发表时间：
2003-03-10
期刊：
影响因子：
6.1
通讯作者：
Lawrence, JF
中科院分区：
文献类型：
--
作者：
Aguete, EC;Gago-Martínez, A;Lawrence, JF
微囊藻毒素起源于淡水中存在的蓝细菌物种，最近被发现是水生环境中痕量的重要污染物。 HPLC 和 HPCE 结合 UV 检测已应用于此类有毒化合物的测定。用于选择性提取和净化微囊藻毒素的免疫亲和层析已成功应用于不同的基质。提出了明确测定这些毒素的简单方案，并将免疫亲和净化与常规使用的固相萃取程序进行了比较。还描述了基于场放大样品堆积的在线预浓缩程序的开发和优化，用于通过 HPCE 在胶束电动色谱模式下分析微囊藻毒素，使用硼酸盐缓冲液和阴离子表面活性剂 SDS 作为分离电解质。结果表明，可以在水样中检测到亚纳克/克含量的微囊藻，而在藻类样品中可以测定亚微克/克浓度。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Microcystins, which have their origin in species of cyanobacteria present in freshwaters, have recently been found to be important contaminants of the aquatic environment at trace levels. HPLC and HPCE with UV detection have been applied in the determination of such toxic compounds. Immunoaffinity chromatography for the selective extraction and clean-up of microcystins has been successfully applied to different matrices. Simple protocols for unambiguous determination of these toxins are presented and the immunoaffinity clean-up is compared with conventionally used solid phase extraction procedures. The development and optimisation of an on-line preconcentration procedure based on field amplified sample stacking for the analysis of microcystins by HPCE in the micellar electrokinetic chromatography mode is also described, using borate buffer with the anionic surfactant SDS, as separation electrolyte. Results indicate that sub-nanogram/gram content of microcyst ins can be detected in water samples, while submicrogram/gram concentrations can be determined in algae samples. (C) 2002 Elsevier Science B.V. All rights reserved.