Characterization of keyhole limpet hemocyanin (KLH) glycans sharing a carbohydrate epitope with Schistosoma mansoni glycoconjugates
Characterization of keyhole limpet hemocyanin (KLH) glycans sharing a carbohydrate epitope with Schistosoma mansoni glycoconjugates
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DOI：
10.1016/j.micron.2003.10.033
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发表时间：
2004-01-01
期刊：
影响因子：
2.4
通讯作者：
Geyer, R
中科院分区：
文献类型：
--
作者：
Geyer, H;Wuhrer, M;Geyer, R
已知匙孔血蓝蛋白（KLH）与曼氏血吸虫共享碳水化合物表位。为了确定观察到的血清学交叉反应性的结构基础，通过酶处理或肼解释放KLH聚糖，并用针对S.曼氏虫卵抗原两个主要的，非反应性的寡糖物种，以及识别的次要化合物分离的二维高效液相色谱法和凝集素亲和色谱法的一部分，并通过质谱法进行表征。结果表明，KLH主要携带高甘露糖型聚糖以及短糖链。作为特征性特征，许多后一种聚糖含有Gal（beta1-6）Man-单元，其尚未在糖蛋白-N-聚糖中发现。与溶酶体聚糖交叉反应的寡聚体包含末端Fuc（α 1 -3）GalNAc基序，其似乎代表介导KLH与来自S的糖缀合物交叉反应的主要碳水化合物表位。mansoni (C)2003爱思唯尔有限公司。保留所有权利。
Keyhole limpet hemocyanin (KLH) is known to share carbohydrate epitopes with Schistosoma mansoni. In order to define the structural basis for the observed serological cross-reactivity, KLH glycans were released either by enzyme treatment or by hydrazinolysis and probed with a rabbit hyperimmune serum directed against S. mansoni egg antigen. Both major, non-reacting oligosaccharide species as well as the minor compounds recognized were isolated by two-dimensional high performance liquid chromatography and in part by lectin affinity chromatography, and characterized by mass spectrometry. The results revealed that KLH carries predominantly high mannose-type glycans as well as short sugar chains. As a characteristic feature, a number of the latter glycans contained a Gal(beta1-6)Man-unit, which has not yet been found in glycoprotein-N-glycans. Oligosaccharides cross-reacting with schistosomal glycans comprised a terminal Fuc(alpha1-3)GalNAc-motif, which appears to represent the main carbohydrate epitope mediating cross-reactivity of KLH with glycoconjugates from S. mansoni. (C) 2003 Elsevier Ltd. All rights reserved.