Pathways of clearance in mouse lungs exposed to iron oxide aerosols
Pathways of clearance in mouse lungs exposed to iron oxide aerosols
复制标题
暴露于氧化铁气溶胶的小鼠肺部的清除途径
DOI：
10.1002/ar.1091810304
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
J. Brain
中科院分区：
文献类型：
--
作者：
S. Sorokin;J. Brain
颗粒清除的历史在小鼠肺中进行了研究，这些小鼠在单次暴露于亚微米颗粒氧化铁气溶胶（用作大气粉尘的类似物）后，每隔14个月连续牺牲一次。清除后，在未染色和普鲁士蓝染色的冷冻和塑料包埋切片中进行光学显微镜检查，以及通过电子显微镜检查氧化铁，其中氧化铁可以通过其形式识别。通过胃肠道和血管途径给铁化合物后肺溶酶体酸性磷酸酶活性的组织化学证明和各种组织的普鲁士蓝染色来研究相关问题。
The history of particle clearance was studied in lungs of mice serially sacrificed at intervals up to 14 months following single exposures to an aerosol of submicronic, particulate, iron oxide used as a similitude for atmospheric dust. Clearance was followed by light microscopy in unstained and Prussian blue stained frozen and plastic embedded sections, as well as by electron microscopy, where iron oxide can be recognized by its form. Related problems were investigated through histochemical demonstration of acid phosphatase activity in pulmonary lysosomes and Prussian blue staining of various tissues after administration of iron compounds by gastrointestinal and vascular routes.