Ebola virus (EBOV) VP24 inhibits transcription and replication of the EBOV genome.
Ebola virus (EBOV) VP24 inhibits transcription and replication of the EBOV genome.
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埃博拉病毒 (EBOV) VP24 抑制 EBOV 基因组的转录和复制。
DOI：
10.1086/520582
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Kawaoka,Yoshihiro
中科院分区：
文献类型：
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作者：
Watanabe,Shinji;Noda,Takeshi;Halfmann,Peter;Jasenosky,Luke;Kawaoka,Yoshihiro
埃博拉病毒（EBOV） VP24在核衣壳（NC）形成中的作用以及VP24在NC形成过程中对病毒基因组转录和复制的影响尚不清楚。因此，我们检测了VP24对EBOV小基因组中报告基因（荧光素酶）、病毒RNA和信使RNA表达的影响。VP24以剂量依赖的方式抑制荧光素酶和这两种rna的表达，表明VP24抑制EBOV基因组的转录和复制。相比之下，flag标记的VP24，不能支持nc样结构的形成，并没有明显降低荧光素酶的表达，这表明VP24与核糖核蛋白复合物的结合是抑制荧光素酶的必要条件。糖蛋白和VP40不影响vp24介导的转录和复制抑制。总之，这些结果表明VP24通过与病毒感染细胞中的核糖核蛋白复合体直接关联来减少EBOV基因组的转录和复制。
The roles of Ebola virus (EBOV) VP24 in nucleocapsid (NC) formation and the effect of VP24 on transcription and replication of the viral genome during NC formation remain unknown. We therefore examined the effect of VP24 on the expression of a reporter gene (luciferase), viral RNA, and messenger RNA from the EBOV minigenome. VP24 inhibited the expression of luciferase and both RNAs in a dose-dependent manner, suggesting that VP24 inhibits transcription and replication of the EBOV genome. By contrast, FLAG-tagged VP24, which cannot support NC-like structure formation, did not appreciably decrease luciferase expression, indicating that association of VP24 with the ribonucleoprotein complex is required for inhibition. Glycoprotein and VP40 did not affect VP24-mediated inhibition of transcription and replication. Together, these results suggest that VP24 reduces transcription and replication of the EBOV genome by direct association with the ribonucleoprotein complex in virus-infected cells.