CANDIDATE CYTOMEGALOVIRUS STRAIN FOR HUMAN VACCINATION
CANDIDATE CYTOMEGALOVIRUS STRAIN FOR HUMAN VACCINATION
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DOI：
10.1128/iai.12.3.521-527.1975
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
HUYGELEN, C
中科院分区：
文献类型：
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作者：
PLOTKIN, SA;FURUKAWA, T;HUYGELEN, C
在WI-38人成纤维细胞培养物中，从一名受感染婴儿的尿液中分离出一种名为Towne的人巨细胞病毒株。然后将其在WI-38中传代125次，包括3次克隆，并在相同的细胞基质中制备池，用作潜在的减毒活疫苗。Towne病毒具有广泛的抗原性，并与AD-169株发生交叉反应。Towne病毒的几个标记被发现，区分它从新鲜的分离物。其中之一是前者对胰蛋白酶的抗性。在准备人体试验时，对Towne病毒进行了无致癌性或其他有害作用的试验。
A strain of human cytomegalovirus called Towne was isolated in WI-38 human fibrolast cell cultures from the urine of an infected infant. It was then passaged 125 times in WI-38, including three clonings, and a pool was prepared in the same cell substrate for use as a potential live attenuated vaccine. The Towne virus has a broad antigenicity and cross-reacts with the AD-169 strain. Several markers of the Towne virus were found which differentiated it from fresh isolates. One of these was resistance of the former to trypsin. The Towne virus was tested for freedom from oncogenicity or other harmful effects in preparation for tests in humans.