CLASSIFICATION OF TRACHOMA VIRUS STRAINS BY PROTECTION OF MICE FROM TOXIC DEATH.
CLASSIFICATION OF TRACHOMA VIRUS STRAINS BY PROTECTION OF MICE FROM TOXIC DEATH.
复制标题
通过保护小鼠免遭中毒性死亡来对沙眼病毒株进行分类。
作者：
San;J. Grayston
从台湾分离的29株沙眼病毒和从世界其他地区获得的9株沙眼病毒和2株包涵性结膜炎病毒在小鼠身上进行了静脉毒性交叉保护试验。这些TRIC病毒的卵黄囊粗悬液用于保护和攻毒。通过在攻毒前14天和7天静脉注射最低致死毒性剂量的三分之一和三分之二来产生保护。所有29个台湾沙眼毒株均属于TW-1和TW-3两个交叉保护组之一。来自新德里的三种沙眼毒株相互保护，并抵御了TW-3组的成员。来自亚利桑那州阿帕奇印第安人的两种沙眼菌株与TW-1组交叉保护。一种沙特阿拉伯沙眼和一种冈比亚沙眼相互保护，但对其他被测试的沙眼菌株不起作用。两株沙眼和两株包涵性结膜炎未能与任何一株交叉保护。因此，展示了七种不同的交叉保护类型。结论是，在小鼠毒性预防试验中，TRIC病毒株的不同组的总数尚不清楚，但在给定的地理区域（如台湾有两个组），不同组的数量是有限的。
Twenty-nine trachoma virus strains isolated on Taiwan and nine trachoma strains and two inclusion conjunctivitis strains obtained from other areas of the world have been tested in mice for cross-protection against intravenous toxicity. Crude yolk sac suspensions of these TRIC viruses were used both for protection and challenge. Protection was produced by intravenous injection of one-third and then two-thirds a minimum lethal toxic dose 14 and 7 days prior to challenge. All 29 of the Taiwan trachoma strains fell into one of two cross-protecting groups designated as the TW-1 and TW-3 groups. Three trachoma strains from New Delhi protected against each other and members of the TW-3 group. Two trachoma strains from Apache Indians in Arizona cross-protected with the TW-1 group. A Saudi Arabia and a Gambia trachoma strain protected against each other but not against the other strains tested. Two trachoma and two inclusion conjunctivitis strains failed to cross-protect with any strain. Therefore, seven different cross-protecting types were demonstrated. It is concluded that the total number of different groups of TRIC virus strains in the mouse toxicity prevention test are not yet known, but that in a given geographic area (as exemplified by Taiwan with two groups) the number of different groups is limited.