Isolation and expression of scabrous, a gene regulating neurogenesis in Drosophila.
Isolation and expression of scabrous, a gene regulating neurogenesis in Drosophila.
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果蝇神经发生调节基因 scabrous 的分离和表达。
DOI：
10.1101/gad.4.11.1848
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发表时间：
1990
影响因子：
10.5
通讯作者：
Gerald M. Rubin
中科院分区：
文献类型：
--
作者：
M. Mlodzik;N. Baker;Gerald M. Rubin
果蝇sca基因的突变影响眼睛和刚毛的发育，导致成虫小眼和刚毛重复的间隔不规则。我们用P-元件标签法克隆了sca基因。sca转录单位为12 kb，由4个外显子组成，它们连接在一个3.2 kb的mRNA中。在增强子陷阱屏幕中，我们已经分离出几个P[lacZ]插入接近SCA转录起始位点。我们已经研究了sca的表达模式，通过原位杂交sca成绩单，β-半乳糖苷酶定位在P[lacZ]线，并通过免疫细胞化学与抗sca抗血清。在胚胎发生期间，sca以与神经发育相关的动态模式表达。在成虫发育过程中，sca主要表达于眼成虫盘的R8感光细胞前体细胞和其它盘的感觉器官前体细胞。在翼盘中，sca表达与刚毛原基同延，并受无毛-盾板复合体基因控制。基于其功能丧失的表型、表达模式及其产物（一种与纤维蛋白原基因家族同源的分泌肽）的预测结构，我们提出sca编码一种参与发育中神经系统各个结构域的侧抑制的信号。
Mutations in the Drosophila scabrous (sca) gene affect eye and bristle development, leading to irregular spacing of ommatidia and bristle duplications in the adult fly. We have cloned the sca gene by P-element tagging. The sca transcription unit is 12 kb and consists of four exons that are joined in a 3.2-kb mRNA. In an enhancer trap screen we have isolated several P[lacZ] insertions close to the sca transcription start site. We have examined the expression pattern of sca by in situ hybridization to sca transcripts, by beta-galactosidase localization in the P[lacZ] lines, and by immunocytochemistry with an anti-sca antiserum. During embryogenesis, sca is expressed in a dynamic pattern associated with neural development. During imaginal development, sca is mainly expressed in the R8 photoreceptor precursor cells in the eye imaginal disc and in sensory organ precursor cells in other discs. In the wing disc, sca expression is coextensive with the anlagen for bristles and is controlled by genes of the achaete-scute complex. Based on its loss-of-function phenotype, expression pattern, and the predicted structure of its product, a secreted peptide with homology to the fibrinogen gene family, we propose that sca encodes a signal involved in lateral inhibition within individual domains of the developing nervous system.