Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla
Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla
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DOI：
10.1016/j.virol.2003.10.033
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发表时间：
2004-02-20
期刊：
影响因子：
3.7
通讯作者：
Bratbak, G
中科院分区：
文献类型：
--
作者：
Brussaard, CPD;Noordeloos, AAM;Bratbak, G
我们报告的第一个双链（ds）RNA病毒在家庭呼肠孤病毒感染原生生物（微单胞菌，Prasinophyceae）的分离。dsRNA基因组由11个片段组成，范围在0.8和5.8 kb之间，总大小约为25.5 kb。该病毒（MpRNAV-01 B）不能被分配到属水平，因为宿主类型，基因组大小和小于2 kb的片段数不对应于两个现有的11节段dsRNA属轮状病毒和水生呼肠孤病毒。MpRNAV-01 B的粒径为65-80 nm，宿主范围窄，潜伏期为36 h，含有5种主要蛋白质（120、95、67、53和32 kDa）。MpRNAV-01 B对氯仿、乙醚、非离子去污剂、螯合剂和还原剂具有良好的抗冻融性。在高于35 ℃的温度下，通过离子去污剂、乙醇、丙酮和酸性条件（pH 2-5）灭活病毒。(C)2004年爱思唯尔公司All rights reserved.
We report the isolation of the first double-stranded (ds) RNA virus in the family Reoviridae that infects a protist (microalga Micromonas pusilla, Prasinophyceae). The dsRNA genome was composed of 11 segments ranging between 0.8 and 5.8 kb, with a total size of approximately 25.5 kb. The virus (MpRNAV-01B) could not be assigned to the genus level because host type, genome size, and number of segments smaller than 2 kb did not correspond to either of the two existing 11-segmented dsRNA genera Rotavirus and Aquareovirus. MpRNAV-01B has a particle size of 65-80 nm, a narrow host range, a latent period of 36 h, and contains five major proteins (120, 95, 67, 53, and 32 kDa). MpRNAV-01B was stable to freeze-thawing, resistant to chloroform, ether, nonionic detergents, chelating and reducing agents. The virus was inactivated at temperatures above 35 degreesC and by ionic detergent, ethanol, acetone, and acidic conditions (pH 2-5). (C) 2004 Elsevier Inc. All rights reserved.