Dynamics of oxytetracycline, sulfamerazine, and ciprofloxacin and related antibiotic resistance genes during swine manure composting
Dynamics of oxytetracycline, sulfamerazine, and ciprofloxacin and related antibiotic resistance genes during swine manure composting
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猪粪堆肥过程中土霉素、磺胺甲基嘧啶和环丙沙星及相关抗生素抗性基因的动态
DOI：
10.1016/j.jenvman.2018.09.074
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发表时间：
2019-01-15
影响因子：
8.7
通讯作者：
Li, Zhaojun
中科院分区：
文献类型：
--
作者：
Cheng, Dengmiao;Feng, Yao;Li, Zhaojun
了解畜禽粪便堆肥过程中抗生素及其相关抗生素耐药基因（ARGs）的动态变化对评价抗生素的环境风险至关重要，可以有效降低抗生素对自然环境的影响。研究了土霉素（OTC）、磺胺甲基嘧啶（SM 1）和环丙沙星（CIP）在猪粪堆肥过程中的消解规律及其相应的ARGs的行为。这些抗生素以两种浓度水平和两种不同的添加方法（单一/混合）添加。结果表明，除单一SM 1处理外，堆肥对抗生素的去除率均在85%以上。四环素耐药基因（TRG）、核糖体保护蛋白（RPP）、外排泵（EFP）和氟喹诺酮耐药基因（FRGS）在42 d后可被有效去除。相反，编码酶失活（EI）和磺胺类药物耐药基因（SRG）的TRG富集高达31倍（单一低SM 1中的sul 2）。统计分析表明，这些类抗生素和ARGs的行为控制的微生物活性和显着的环境因素（主要是C/N，水分和pH值）在整个堆肥过程中的影响。
Understanding the dynamics of veterinary antibiotic and related antibiotic resistance genes (ARGs) during swine manure composting is crucial in assessing the environmental risk of antibiotics, which could effectively reduce their impact in natural environments. This study investigated the dissipation of oxytetracycline (OTC), sulfamerazine (SM1) and ciprofloxacin (CIP) as well as the behaviour of their corresponding ARGs during swine manure composting. These antibiotics were added at two concentration levels and two different methods of addition (single/mixture). The results indicated that the removal efficiency of antibiotics by composting were >= 85%, except for the single-SM1 treatment. The tetracycline resistance genes (TRGs) encoding ribosomal protection proteins (RPP) and efflux pump (EFP) and fluoroquinolone resistance genes (FRGS) could be effectively removed after 42 days. On the contrary, the TRGs encoding enzymatic inactivation (EI) and sulfonamide resistance genes (SRGs) were enriched up to 31-fold (sul2 in single-low-SM1). Statistical analyses indicated that the behaviour of these class antibiotics and ARGs were controlled by microbial activity and significantly influenced by environmental factors (mainly C/N, moisture and pH) throughout the composting process.